Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 9

The DOI matches its Crossref record.

Epstein-Barr Virus- (EBV-) Immortalized Lymphoblastoid Cell Lines (LCLs) Express High Level of CD23 but Low CD27 to Support Their Growth

Yap, Hooi-Yeen, Siow, Thin-Sam, Chow, Sook-Khuan, Teow, Sin-Yeang·2019·Advances in Virology
DOI 10.1155/2019/6464521PMC PMC6458955
Open this paper in CoChat — free account

Abstract

Epstein-Barr virus (EBV) is one of the common human herpesvirus types in the world. EBV is known to infect more than 95% of adults in the world. The virus mainly infects B lymphocytes and could immortalize and transform the cells into EBV-bearing lymphoblastoid cell lines (LCLs). Limited studies have been focused on characterizing the surface marker expression of the immortalized LCLs. This study demonstrates the generation of 15 LCLs from sixteen rheumatoid arthritis (RA) patients and a healthy volunteer using B95-8 marmoset-derived EBV. The success rate of LCL generation was 88.23%. All CD19+ LCLs expressed CD23 (16.94-58.9%) and CD27 (15.74-80.89%) on cell surface. Our data demonstrated two distinct categories of LCLs (fast- and slow-growing) (p<0.05) based on their doubling time. The slow-growing LCLs showed lower CD23 level (35.28%) compared to fast-growing LCLs (42.39%). In contrast, the slow-growing LCLs showed higher percentage in both CD27 alone and CD23+CD27+ in combination. Overall, these findings may suggest the correlations of cellular CD23 and CD27 expression with the proliferation rate of the generated LCLs. Increase expression of CD23 may play a role in EBV immortalization of B-cells and the growth and maintenance of the EBV-transformed LCLs while CD27 expression might have inhibitory effects on LCL proliferation. Further investigations are warranted to these speculations.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1155/2019/6464521

Cites

Statistics

Figures

Figure 1 Microscopic examination of PBMC-derived EBV-immortalized B-cells from a RA patient 8A one week after infection. These immortalized cells are annotated as LCL-8A. Red arrows show the rosette-like EBV-immortalized LCLs at 4x (upper panel), 10x (middle panel), and 20x (bottom panel) magnifications. Scale bars are indicated in yellow.
Figure 1 Microscopic examination of PBMC-derived EBV-immortalized B-cells from a RA patient 8A one week after infection. These immortalized cells are annotated as LCL-8A. Red arrows show the rosette-like EBV-immortalized LCLs at 4x (upper panel), 10x (middle panel), and 20x (bottom panel) magnifications. Scale bars are indicated in yellow.CC BY · PubMed Central
Figure 2 Growth curves of LCLs. Relative luminescence units represent the viability signals of cells at various time points. The filled lines represent the fast-growing LCLs whereas dotted lines represent the slow-growing LCLs.
Figure 2 Growth curves of LCLs. Relative luminescence units represent the viability signals of cells at various time points. The filled lines represent the fast-growing LCLs whereas dotted lines represent the slow-growing LCLs.CC BY · PubMed Central
Figure 3 T-cell (CD3) and B-cell (CD19) surface marker profiling by flow cytometry. The upper panel shows the analysis of PBMCs isolated from patient 8A while the lower panel shows the analysis of the corresponding LCLs (LCL-8A). R1: 7AAD− live cells; R2: gated lymphocytes; R3: gated CD3+ T-cells; R4: gated CD19+ B-cells.
Figure 3 T-cell (CD3) and B-cell (CD19) surface marker profiling by flow cytometry. The upper panel shows the analysis of PBMCs isolated from patient 8A while the lower panel shows the analysis of the corresponding LCLs (LCL-8A). R1: 7AAD− live cells; R2: gated lymphocytes; R3: gated CD3+ T-cells; R4: gated CD19+ B-cells.CC BY · PubMed Central
Figure 4 CD23 and CD27 expression analysis of CD19+ B-cells by flow cytometry. The upper panel shows the analysis of PBMCs isolated from patient 8A while the lower panel shows the analysis of the corresponding LCLs (LCL-8A). R3: gated CD27+ cells; R4: gated CD19+ B-cells; R5: gated CD19+CD23+; R6: gated CD23+CD27+ cells; R7: gated CD19+CD27+ cells; R8: gated CD19+CD23+CD27+ cells.
Figure 4 CD23 and CD27 expression analysis of CD19+ B-cells by flow cytometry. The upper panel shows the analysis of PBMCs isolated from patient 8A while the lower panel shows the analysis of the corresponding LCLs (LCL-8A). R3: gated CD27+ cells; R4: gated CD19+ B-cells; R5: gated CD19+CD23+; R6: gated CD23+CD27+ cells; R7: gated CD19+CD27+ cells; R8: gated CD19+CD23+CD27+ cells.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 19

  1. 10.12688/f1000research.10591.1 · DOI
  2. 10.1128/JVI.01677-16 · DOI
  3. 2015, Experimental and Molecular Medicine, 47, e130, 10.1038/emm.2014.109 · DOI
  4. 2017, Scientific Reports, 7
  5. 2012, International Journal of Molecular and Cellular Medicine, 1, 75
  6. 10.1586/14760584.7.4.411 · DOI
  7. 10.3109/1547691X.2015.1132288 · DOI
  8. 10.3390/cells7100161 · DOI
  9. 10.1097/BOR.0b013e3283369cb8 · DOI
  10. 2001, The Journal of Rheumatology, 28, 1222
  11. 10.4049/jimmunol.1203366 · DOI
  12. 2018, Frontiers in Immunology, 9, 626, 10.3389/fimmu.2018.00626 · DOI
  13. 2012, Arthritis Care and Research, 64, 640, 10.1002/acr.21649 · DOI · Paper page
  14. 10.3791/3321 · DOI
  15. 10.1073/pnas.84.10.3452 · DOI
  16. 2000, Immunology Today, 21, 204, 10.1016/S0167-5699(00)01605-4 · DOI
  17. 10.1371/journal.pone.0004834 · DOI
  18. 10.1099/0022-1317-71-3-665 · DOI
  19. 2011, Virology Journal, 8

Cited by

See all 9

Metadata

Authors
Yap, H.-Y., Siow, T.-S., Chow, S.-K., & Teow, S.-Y.
Affiliations
  • Department of Medical Sciences, School of Healthcare and Medical Sciences, Sunway University, Jalan Universiti, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia
  • Department of Biological Sciences, School of Science and Technology, Sunway University, Jalan Universiti, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia
  • Sunway Medical Centre, Jalan Lagoon Selatan, Bandar Sunway, 47500 Subang Jaya, Selangor Darul Ehsan, Malaysia
ORCID
Teow, Sin-Yeang
Venue
Advances in Virology
Publisher
Wiley
Published
2019-03-28
Volume
2019
Pages
1-9
ISSN
  • 1687-8639
  • 1687-8647
DOI
10.1155/2019/6464521
PMC
PMC6458955
Cited by
9
References
0
Language
en
Licence
creativecommons.org/licenses/by/4.0/ (unspecified)
Funders
  • Sunway University (INT-2019-SHMS-DMS-01, EXT-SIDS-SIHD-MAKNA-2017-01, SRC/002/2017/FR and SRC/003/2017/FR)
  • Majlis Kanser Nasional (INT-2019-SHMS-DMS-01, EXT-SIDS-SIHD-MAKNA-2017-01, SRC/002/2017/FR and SRC/003/2017/FR)
  • Sunway Medical Centre Research Funds (INT-2019-SHMS-DMS-01, EXT-SIDS-SIHD-MAKNA-2017-01, SRC/002/2017/FR and SRC/003/2017/FR)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Epstein-Barr Virus- (EBV-) Immortalized Lymphoblastoid Cell Lines (LCLs) Express High Level of CD23 but Low CD27 to Support Their Growth. https://app.cochat.ai/papers/10.1155/2019/6464521
The paper
Yap, H.-Y., Siow, T.-S., Chow, S.-K., & Teow, S.-Y. (2019). Epstein-Barr Virus- (EBV-) Immortalized Lymphoblastoid Cell Lines (LCLs) Express High Level of CD23 but Low CD27 to Support Their Growth. Advances in Virology. https://doi.org/10.1155/2019/6464521