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Cited by 52Bee Products Chemical AnalysisHealthcare and Venom ResearchPhytochemicals and Antioxidant Activities

Determination of Total Phenolics, Flavonoids, and Antioxidant Activity and GC-MS Analysis of Malaysian Stingless Bee Propolis Water Extracts

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Syed Salleh, Sharifah Nur Amalina, Mohd Hanapiah, Nur Ayuni, Ahmad, Hafandi, Wan Johari, Wan Lutfi, Osman, Nurul Huda, Mamat, Mohd Razif·2021·Scientifica
DOI 10.1155/2021/3789351PMC PMC8556095
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Abstract

Propolis contains polyphenolic compounds such as flavonoids and phenols that are able to demonstrate a broad spectrum of biological activities including antioxidant, antibacterial, and many more. This study was carried out to determine the total phenolics, flavonoids, and antioxidant activity of water-extracted propolis samples from three different Indo-Malayan stingless bee species, namely, Tetrigona apicalis, Tetrigona binghami, and Homotrigona fimbriata. Total phenolic and flavonoid contents were evaluated using Folin–Ciocalteu colorimetric and aluminium chloride methods, respectively, while the antioxidant activity was analysed using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay. Results indicated that H. fimbriata extracts exhibit the highest TPC, TFC, and antiradical activity among all samples tested. Interestingly, the data also showed that the higher the concentration of the extract used, the higher the antioxidant activity exhibited by the samples. Statistically, there were no significant differences recorded between the different bee species’ propolis studied. In conclusion, the propolis extracts showed stronger antioxidant potential with higher TPC and TFC values. This study also noted the presence of bioactive compounds from local stingless bee propolis that could potentially be utilised for their medicinal and health benefits.

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  1. [1]Kraikongjit S. Jongjitvimol T. Mianjinda N. Antibacterial effect of plant resin collected from Tetrigona apicalis (Smith, 1857) in Thung Salaeng Luang national park, Phitsanulok Journal of Science and Technology 2018 15 8 599 607
  2. [2]Smyk P. Hołyńska-Iwan I. Olszewska-Słonina D. Effect of propolis preparations on transepithelial electrical potential, resistance, and ion transport in in vitro study Evidence-Based Complementary and Alternative Medicine 2019 2019 9 3756092 10.1155/2019/3756092 2-s2.0-85060806237 PMC6350614 30766612 · DOI
  3. [3]Popova M. Trusheva B. Bankova V. Propolis of stingless bees: a phytochemist’s guide through the jungle of tropical biodiversity Phytomedicine 2019 86 1 52 153098 31648904
  4. [4]Suib M. S. M. Omar W. A. W. Omar E. A. Mohamed R. Ethanolic extract of propolis from the Malaysian stingless bee Geniotrigona thoracica inhibits formation of THP-1 derived macrophage foam cells Journal of Apicultural Research 2020 60 3 478 490 10.1080/00218839.2020.1720125 · DOI
  5. [5]Georgieva K. Popova M. Dimitrova L. Phytochemical analysis of Vietnamese propolis produced by the stingless bee Lisotrigona cacciae PLoS One 2019 14 4 e0216074 10.1371/journal.pone.0216074 2-s2.0-85064874437 PMC6481864 31017965 · DOI
  6. [6]Ibrahim N. Mohd Niza N. F. S. Mohd Rodi M. M. Zakaria A. J. Ismail Z. Mohd K. S. Chemical and biological analyses of Malaysian stingless bee propolis extracts Malaysian Journal of Analytical Science 2016 20 2 413 422 10.17576/mjas-2016-2002-26 2-s2.0-84964213239 · DOI
  7. [7]Kasote D. M. Pawar M. V. Gundu S. S. Chemical profiling, antioxidant, and antimicrobial activities of Indian stingless bees propolis samples Journal of Apicultural Research 2019 58 4 617 625 10.1080/00218839.2019.1584960 2-s2.0-85063226046 · DOI
  8. [8]Wong P. Hii S. L. Koh C. C. Moh T. S. Y. Gindi S. R. A. Chemical analysis on the honey of Heterotrigona itama and Tetrigona binghami from Sarawak, Malaysia Sains Malaysiana 2019 48 8 1635 1642 10.17576/jsm-2019-4808-09 2-s2.0-85073718592 · DOI
  9. [10]Awang N. Ali N. Majid F. A. A. Hamzah S. Razak S. B. A. Total flavonoids and phenolic contents of sticky and hard propolis from 10 species of Indo-Malayan stingless bees Malaysian Journal of Analytical Sciences 2018 22 5 877 884
  10. [11]Akhir R. A. Bakar A. M. F. Shuaibu B. S. Antioxidant and antimicrobial activity of stingless bee bread and propolis extracts Proceedings of the 2nd International Conference On Applied Science And Technology 2017 October 2017 Brussels, Belgium
  11. [12]Mohamed W. A. S. Ismail N. Z. Omar E. A. Abdul Samad N. Adam S. K. Mohamad S. GC-MS evaluation, antioxidant content, and cytotoxic activity of propolis extract from peninsular Malaysian stingless bees, Tetrigona apicalis Evidence-Based Complementary and Alternative Medicine 2020 2020 9 8895262 10.1155/2020/8895262 PMC7759394 33381215 · DOI
  12. [13]Ahmad Tarmizi Wan Yusop S. Hafizi Sukairi A. Wan Sabri W. M. A. Razip Asaruddin M. Antioxidant, antimicrobial and cytotoxicity activities of propolis from beladin, sarawak stingless bees Trigona itama extract Materials Today: Proceedings 2019 19 1752 1760 10.1016/j.matpr.2019.11.213 · DOI
  13. [14]Pazin W. M. Mônaco L. d. M. Egea Soares A. E. Miguel F. G. Berretta A. A. Ito A. S. Antioxidant activities of three stingless bee propolis and green propolis types Journal of Apicultural Research 2017 56 1 40 49 10.1080/00218839.2016.1263496 2-s2.0-85009961980 · DOI
  14. [15]Campos J. F. Santos U. P. d. Rocha P. d. S. d. Antimicrobial, antioxidant, anti-inflammatory, and cytotoxic activities of propolis from the stingless bee Tetragonisca fiebrigi (jataí) Evidence-Based Complementary and Alternative Medicine 2015 2015 11 296186 10.1155/2015/296186 2-s2.0-84936797696 PMC4491730 26185516 · DOI
  15. [16]Tran T. D. Ogbourne S. M. Brooks P. R. Sánchez-Cruz N. Medina-Franco J. L. Quinn R. J. Lessons from exploring chemical space and chemical diversity of propolis components International Journal of Molecular Sciences 2020 21 4988 1 35 10.3390/ijms21144988 PMC7404124 32679731 · DOI
  16. [18]Pobiega K. Kraśniewska K. Derewiaka D. Gniewosz M. Comparison of the antimicrobial activity of propolis extracts obtained by means of various extraction methods Journal of Food Science & Technology 2019 56 12 5386 5395 10.1007/s13197-019-04009-9 2-s2.0-85073956206 31749486 PMC6838241 · DOI
  17. [19]Kubiliene L. Laugaliene V. Pavilonis A. Alternative preparation of propolis extracts: comparison of their composition and biological activities BMC Complementary and Alternative Medicine 2015 15 156 156 157 10.1186/s12906-015-0677-5 2-s2.0-84938949803 26012348 PMC4443635 · DOI · Paper page
  18. [20]Awanis Ainur M. B. Nur Basyirah Z. Aulia Rani A. Nor Elai M. N. Khamsah Suryati M. Phytochemical screening and antioxidant properties of stingless bee Geniotrigona thoracica propolis Malaysian Journal of Fundamental and Applied Sciences 2019 15 330 335
  19. [21]Hernández Zarate M. S. Abraham Juárez M. d. R. Cerón García A. Flavonoids, phenolic content, and antioxidant activity of propolis from various areas of Guanajuato, Mexico Food Science & Technology 2018 38 2 210 215 10.1590/fst.29916 2-s2.0-85049432983 · DOI
  20. [22]Mokhtar S. U. Hooi H. S. Lene D. T. T. Jayaraman S. Comparison of total phenolic and flavonoids contents in Malaysian propolis extract with two different extraction solvents International Journal of Engineering Technology and Sciences 2019 6 2 1 11 10.15282/ijets.v6i2.2577 · DOI
  21. [23]Sena-Lopes Â. Bezerra F. S. B. das Neves R. N. Chemical composition, immunostimulatory, cytotoxic and antiparasitic activities of the essential oil from Brazilian red propolis PLoS One 2018 13 2 1 16 10.1371/journal.pone.0191797 2-s2.0-85041283653 PMC5794096 29390009 · DOI
  22. [24]Fikri A. M. Sulaeman A. Marliyati S. A. Fahrudin M. Antioxidant activity and total phenolic content of stingless bee propolis from Indonesia Journal of Apicultural Science 2019 63 1 139 147 10.2478/jas-2019-0012 2-s2.0-85068401645 · DOI
  23. [25]Mohd Badiazaman A. A. Md Zin N. B. Annisava A. R. Mat Nafi N. E. Mohd K. S. Phytochemical screening and antioxidant properties of stingless bee Geniotrigona thoracica propolis Malaysian Journal of Fundamental and Applied Sciences 2019 15 2-1 330 335 10.11113/mjfas.v15n2-1.1557 · DOI
  24. [26]Ramón-Sierra J. Peraza-López E. Rodríguez-Borges R. Yam-Puc A. Madera-Santana T. Ortiz-Vázquez E. Partial characterization of ethanolic extract of Melipona beecheii propolis and in vitro evaluation of its antifungal activity Revista Brasileira de Farmacognosia 2019 29 3 319 324 10.1016/j.bjp.2019.04.002 2-s2.0-85066821071 · DOI
  25. [27]Asem N. Abdul Gapar N. A. Abd Hapit N. H. Omar E. A. Correlation between total phenolic and flavonoid contents with antioxidant activity of Malaysian stingless bee propolis extract Journal of Apicultural Research 2019 59 437 442 10.1080/00218839.2019.1684050 · DOI
  26. [28]Abdullah N. A. Ja’afar F. Yasin H. M. Physicochemical analyses, antioxidant, antibacterial, and toxicity of propolis particles produced by stingless bee Heterotrigona itama found in Brunei Darussalam Heliyon 2019 5 9 e02476 10.1016/j.heliyon.2019.e02476 2-s2.0-85072163671 PMC6819780 31687571 · DOI
  27. [29]Rosli N. L. Roslan H. Omar E. A. Mokhtar N. Hapit N. H. A. Asem N. Phytochemical analysis and antioxidant activities of Trigona apicalis propolis extract 1791 Proceedings of the 1st Translational Craniofacial Conference 2016 December 2016 Pinang, Malaysia 10.1063/1.4968873 2-s2.0-85008675564 · DOI
  28. [30]Mat Nafi N. E. Md Zin N. B. Pauzi N. Cytotoxicity, antioxidant and phytochemical screening of propolis extracts from four different Malaysian stingless bee species Malaysian Journal of Fundamental and Applied Sciences 2019 15 2-1 307 312 10.11113/mjfas.v15n2-1.1542 · DOI
  29. [31]Alotaibi A. Ebiloma G. U. Williams R. European propolis is highly active against trypanosomatids including Crithidia fasciculata Scientific Reports 2019 9 1 11364 11410 10.1038/s41598-019-47840-y 2-s2.0-85070224978 31388043 PMC6684629 · DOI
  30. [32]Drescher N. Klein A. M. Schmitt T. Leonhardt S. D. A clue on bee glue: new insight into the sources and factors driving resin intake in honeybees (Apis mellifera) PLoS One 2019 14 2 e0210594 10.1371/journal.pone.0210594 2-s2.0-85061145463 PMC6364881 30726258 · DOI
  31. [33]Cheng M. Z. S. Z. Ismail M. Chan K. W. Comparison of sugar content, mineral elements and antioxidant properties of Heterotrigona itama honey from suburban and forest in Malaysia Malaysian Journal of Medicine and Health Sciences 2019 15 SP1 104 112
  32. [34]Liu Y. Han S.-L. Luo Y. Impaired peroxisomal fat oxidation induces hepatic lipid accumulation and oxidative damage in nile tilapia Fish Physiology and Biochemistry 2020 46 4 1229 1242 10.1007/s10695-020-00785-w 32144523 · DOI
  33. [35]Zeng J. Deng S. Wang Y. Li P. Tang L. Pang Y. Specific inhibition of acyl-CoA oxidase-1 by an acetylenic acid improves hepatic lipid and reactive oxygen species (ROS) metabolism in rats fed a high fat diet Journal of Biological Chemistry 2017 292 9 3800 3809 10.1074/jbc.M116.763532 2-s2.0-85014531474 PMC5339762 28077576 · DOI
  34. [36]Ramnath S. Venkataramegowda S. Singh C. Chemical composition of bee propolis collected from different regions in India by GCMS analysis International Journal of Pharmacognosy and Phytochemistry 2015 30 1 1319 1328
  35. [37]Bankova V. Popova M. Trusheva B. Propolis volatile compounds: chemical diversity and biological activity: a review Chemistry Central Journal 2014 8 p. 28 10.1186/1752-153X-8-28 2-s2.0-84899944911 PMC4014088 24812573 · DOI
  36. [39]Sawicka D. Car H. Borawska M. H. Nikliński J. The anticancer activity of propolis Folia Histochemica et Cytobiologica 2012 50 1 25 37 10.5603/fhc.2012.0004 2-s2.0-84860307404 22532133 · DOI
  37. [41]Kurek-Górecka A. Rzepecka-Stojko A. Górecki M. Stojko J. Sosada M. Świerczek-Zięba G. Structure and antioxidant activity of polyphenols derived from propolis Molecules 2014 19 1 78 101 PMC6271064 24362627
  38. [42]Rocha B. A. Bueno P. C. P. Vaz M. M. d. O. L. L. Evaluation of a propolis water extract using a reliable RP-HPLC methodology and in vitro and in vivo efficacy and safety characterisation Evidence-Based Complementary and Alternative Medicine 2013 2013 11 670451 10.1155/2013/670451 2-s2.0-84877957194 PMC3655582 23710228 · DOI
  39. [43]Kubiliene L. Jekabsone A. Zilius M. Comparison of aqueous, polyethylene glycol-aqueous and ethanolic propolis extracts: antioxidant and mitochondria modulating properties BMC Complementary and Alternative Medicine 2018 18 165 165 210 10.1186/s12906-018-2234-5 2-s2.0-85047490152 29792194 PMC5966891 · DOI

Cited by

Sorted by citations

Authors
Syed Salleh, S. N. A., Mohd Hanapiah, N. A., Ahmad, H., Wan Johari, W. L., Osman, N. H., & Mamat, M. R.
Affiliations
  • Faculty of Forestry and Environment, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor Darul Ehsan, Malaysia
  • Universiti Putra Malaysia (MY)
  • Malaysia Genome Institute (MY)
ORCID
Venue
Scientifica
Publisher
Wiley
Published
2021-10-22
Volume
2021
Pages
1-11
ISSN
2090-908X (ISSN-L)
DOI
10.1155/2021/3789351
PMC
PMC8556095
OpenAlex
W3206875502
Cited by
52
References
0
Citations by year
2026: 5 · 2025: 11 · 2024: 15 · 2023: 11 · 2022: 8 · 2021: 1 · 2020: 1
FWCI
6.02
Type
journal article
Language
en
Open access
Licence
Locations
  • Scientifica (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Greater South Information System
Funders
Universiti Kebangsaan Malaysia
Topics
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Bee Products Chemical Analysis
  • Health Sciences › Medicine › Pharmacology › Healthcare and Venom Research
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
Keywords
stingless bee propolis, total phenolic content, total flavonoid content, antioxidant activity, DPPH radical scavenging, aqueous extract, bioactive compounds

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