Cited by 156Angiogenesis and VEGF in CancerLymphatic System and DiseasesAxon Guidance and Neuronal Signaling

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Vascular Endothelial Growth Factor (VEGF)-A165b Is a Weak In vitro Agonist for VEGF Receptor-2 Due to Lack of Coreceptor Binding and Deficient Regulation of Kinase Activity

Kawamura, Harukiyo, Li, Xiujuan, Harper, Steven J., Bates, David O., Claesson-Welsh, Lena·2008·Cancer Research

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Abstract

Vascular endothelial growth factor (VEGF)-A165b is a COOH-terminal splice variant of VEGF-A that has been implicated in negative regulation of angiogenesis. We compared the properties of VEGF-A165b with those of VEGF-A121, VEGF-A145, and VEGF-A165. Induction of tyrosine phosphorylation sites in VEGFR-2 differed between the VEGF ligands as determined by tryptic phosphopeptide mapping and by use of phosphosite-specific antibodies. VEGF-A165b was considerably poorer in inducing phosphorylation of the positive regulatory site Y1052 in VEGFR-2. Whereas this did not affect activation of VEGFR-2 in vitro, we show that VEGF-A165b failed to induce vasculogenesis and sprouting angiogenesis in differentiating embryonic stem cells and vascularization of s.c. Matrigel plugs. In addition, the ability of the different VEGF ligands to induce angiogenesis correlated with their abilities to bind the VEGF coreceptor neuropilin 1 (NRP1). Our data indicate that loss of VEGFR-2/NRP1 complex formation and Y1052 phosphorylation contribute to the lack of angiogenic properties of VEGF-A165b. [Cancer Res 2008;68(12):4683–92]

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Authors: Kawamura, H., Li, X., Harper, S. J., Bates, D. O., & Claesson-Welsh, L.
Affiliations: Uppsala University (SE)
University of Bristol (GB)
1Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden; and
ORCID: Li, Xiujuan
Harper, Steven J.
Bates, David O.
Venue: Cancer Research
Publisher: American Association for Cancer Research (AACR)
Published: 2008-06-15
Volume: 68
Issue: 12
Pages: 4683-4692
ISSN: 0008-5472 (ISSN-L)
1538-7445
DOI: 10.1158/0008-5472.can-07-6577
OpenAlex: W2067020920
Cited by: 156
References: 31
Citations by year: 2025: 4 · 2024: 3 · 2023: 5 · 2022: 8 · 2021: 6 · 2020: 4 · 2019: 10 · 2018: 13 · 2017: 11 · 2016: 10 · 2015: 11 · 2014: 14 · 2013: 9 · 2012: 11
FWCI: 5.08
Type: journal article
Language: en
Open access: closed
Locations: Cancer Research (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
Health Sciences › Medicine › Oncology › Lymphatic System and Diseases
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
Keywords: VEGF-A165b, VEGF splice variants, VEGF receptor 2, neuropilin-1, tyrosine phosphorylation, kinase regulation, angiogenesis, vasculogenesis, sprouting angiogenesis, embryonic stem cells
MeSH terms: Animals, Aorta, Binding, Competitive, Cell Differentiation, Cells, Cultured, Chemotaxis, Collagen, Drug Combinations, Embryo, Mammalian, Endothelium, Vascular, Female, Gene Expression Regulation, Heparitin Sulfate, Heparan Sulfate, Humans, Laminin, Mice, Nude, Phosphorylation, Protein Binding, Proteoglycans, Swine, Tyrosine, Immunoblotting, Signal Transduction, Neovascularization, Physiologic, Neuropilin-1, Vascular Endothelial Growth Factor Receptor-2, Vascular Endothelial Growth Factor A, Immunoprecipitation, Mice

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Vascular Endothelial Growth Factor (VEGF)-A165b Is a Weak In vitro Agonist for VEGF Receptor-2 Due to Lack of Coreceptor Binding and Deficient Regulation of Kinase Activity

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