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Dysregulation of the mitogen granulin in human cancer through the miR-15/107 microRNA gene group

Wang, Wang-Xia, Kyprianou, Natasha, Wang, Xiaowei, Nelson, Peter T.·2010·Cancer Research
DOI 10.1158/0008-5472.can-10-1684PMC PMC2982932
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Abstract

Granulin (GRN) is a potent mitogen and growth factor implicated in many human cancers, but its regulation is poorly understood. Recent findings indicate that GRN is regulated strongly by the microRNA miR-107, which functionally overlaps with miR-15, miR-16, and miR-195 due to a common 5′ sequence critical for target specificity. In this study, we queried whether miR-107 and paralogs regulated GRN in human cancers. In cultured cells, anti-argonaute RNA coimmunoprecipitation with downstream microarray analyses indicates that GRN mRNA is directly targeted by numerous miR-15/107 miRNAs. We further tested this association in human tumors. MiR-15 and miR-16 are known to be downregulated in chronic lymphocytic leukemia (CLL). Using pre-existing microarray datasets, we found that GRN expression is higher in CLL relative to nonneoplastic lymphocytes (P < 0.00001). By contrast, other prospective miR-15/miR-16 targets in the dataset (BCL-2 and cyclin D1) were not upregulated in CLL. Unlike in CLL, GRN was not upregulated in chronic myelogenous leukemia (CML) where miR-107 paralogs are not known to be dysregulated. Prior studies have shown that GRN is also upregulated, and miR-107 downregulated, in prostate carcinoma. Our results indicate that multiple members of the miR-107 gene group indeed repress GRN protein levels when transfected into prostate cancer cells. At least a dozen distinct types of cancer have the pattern of increased GRN and decreased miR-107 expression. These findings indicate for the first time that the mitogen and growth factor GRN is dysregulated via the miR-15/107 gene group in multiple human cancers, which may provide a potential common therapeutic target. Cancer Res; 70(22); 9137–42. ©2010 AACR.

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Sorted by citations

Authors
Wang, W.-X., Kyprianou, N., Wang, X., & Nelson, P. T.
Affiliations
Authors' Affiliations: 1Department of Pathology and Division of Neuropathology, University of Kentucky Medical Center and Sanders-Brown Center on Aging, University of Kentucky, Lexington, Kentucky and 2Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri
Venue
Cancer Research
Publisher
American Association for Cancer Research (AACR)
Published
2010-11-14
Volume
70
Issue
22
Pages
9137-9142
ISSN
  • 0008-5472
  • 1538-7445
DOI
10.1158/0008-5472.can-10-1684
PMC
PMC2982932
Cited by
40
References
0
Language
en

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