Mechanisms of Androgen Induction of Sebocyte Differentiation
Abstract
It has been difficult to induce the expected sebocyte differentiation in vitro with dihydrotestosterone (DHT). We reasoned that our culture system lacks differentiating factors, and peroxisome proliferator-activated receptors (PPARs) were the prime candidates. We tested PPAR activators informative about diverse PPAR subtypes, with and without DHT (10–6 M): BRL-49653 (10–6 M, PPAR-γ), WY-14643 (10–6 M, PPAR-α), and linoleic acid (LIN, 10–4 M, PPAR-d). Treatments were added in serum-free medium to cultures of rat preputial sebocytes. Control, DHT, BRL and BRL + DHT treatments caused 11, 25, 66 and 80%, respectively, of preputial cell colonies to differentiate into lipid-forming colonies (LFCs) (p <0.001). WY induced 20% and LIN over 95% LFC formation. PPAR-γ mRNA was identified in preputial sebocytes by the RNase protection assay. These data suggest that differentiation of sebocytes is transduced by PPARs and have implications for the development of new treatments for acne.
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Linoleic acid is known to activate PPAR-ß.
“As a first step, we administered the potent dihydrotestosterone (DHT) androgen, the PPAR-γ BRL-49653 (BRL) activator and linoleic acid (LIN) known to activate PPAR-ß36.”
From Blimp1+ cells generate functional mouse sebaceous gland organoids in vitro (2019) · cites this paper as [36]
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References 29 · Cited by 156
- Authors
- Rosenfield, R. L., Deplewski, D., Kentsis, A., & Ciletti, N.
- Affiliations
- University of Chicago (US)
- Venue
- Dermatology
- Publisher
- S. Karger AG
- Published
- 1998
- Volume
- 196
- Issue
- 1
- Pages
- 43-46
- ISSN
- 1018-8665 (ISSN-L)
- 1421-9832
- OpenAlex
- W2003525762
- Cited by
- 156
- References
- 29
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 3 · 2021: 3 · 2020: 1 · 2019: 6 · 2018: 5 · 2017: 6 · 2016: 5 · 2015: 13 · 2014: 5 · 2013: 3 · 2012: 3
- FWCI
- 2.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Dermatology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 06308, R01HD006308)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Health Sciences › Medicine › Dermatology › Acne and Rosacea Treatments and Effects
- Health Sciences › Medicine › Dermatology › Dermatology and Skin Diseases
- Keywords
- sebocyte differentiation, androgen induction, dihydrotestosterone, peroxisome proliferator-activated receptors, PPARγ, PPARα, PPARδ, linoleic acid, Wy-14,643, acne treatment
- MeSH terms
- Rosiglitazone, Androgens, Animals, Cell Differentiation, Cells, Cultured, Lipids, Pyrimidines, Sebaceous Glands, Dihydrotestosterone, Thiazoles, Transcription Factors, Receptors, Cytoplasmic and Nuclear, Linoleic Acid, Thiazolidinediones, Rats
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