Neurotrophic Factor Expression after Focal Brain Ischemia Preceded by Different Preconditioning Strategies
Abstract
Background: Both prolonged brain ischemia and preconditioning (PC) induce expression of neurotrophic factors. However, the influence of PC on their expression after a long-term ischemia remains vague. Previously, we have found various effects of PC on mRNA levels of different cytokines after focal brain ischemia. Thus, we investigated mRNA expression of nerve growth factor, brain-derived neurotrophic factor (BDNF) and ciliary neurotrophic factor after 90-min middle cerebral artery occlusion (MCAo) preceded by ischemic or chemical PC. Methods: MCAo was induced in rats using the suture method. PC had been carried out 3 days earlier. There were 4 experimental groups: MCAo alone; ischemic PC and MCAo; chemical PC and MCAo, and sham-operated rats. Expression of mRNAs in the ipsi- and contralateral cortex was studied by semiquantitative RT-PCR at 12 and 24 h after MCAo. Results: Despite clearly neuroprotective effects of both PC strategies, mRNA levels of neurotrophic factors were similar in tolerant and nontolerant rats. Only BDNF mRNA expression, 12 h after reperfusion, was lower when ischemic PC was applied prior to long-term ischemia. Conclusions: These results suggest that PC generally does not change the expression of neurotrophic factor expression after a long-term focal brain ischemia compared to the nontolerant state.
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The upregulation of Bdnf transcription in the ischemic cortex, including the damaged area and periinfarction zone, was shown in our study to correspond with other evidence.
“The upregulation of Bdnf transcription in the ischemic cortex, including the damaged area and periinfarction zone, was shown in our study to correspond with other evidence (Kokaia et al. 1995; Ferrer et al. 2001; Pera et al. 2005; Kim et al. 2005).”
From Semax and Pro-Gly-Pro activate the transcription of neurotrophins and their receptor genes after cerebral ischemia (2010) · cites this paper as Pera et al. 2005
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Dmitrieva, V. G., Povarova, O. V., Skvortsova, V. I., Limborska, S. A., Myasoedov, N. F., & Dergunova, L. V. (2010) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-009-9432-0 · Cited by 54 · see citing works
- Authors
- Pera, J., Zawadzka, M., Kaminska, B., & Szczudlik, A.
- Affiliations
- Jagiellonian University (PL)
- Instytut Biologii Doświadczalnej im. Marcelego Nenckiego (PL)
- ORCID
- Pera, Joanna
- Venue
- Cerebrovascular Diseases
- Publisher
- S. Karger AG
- Published
- 2005
- Volume
- 19
- Issue
- 4
- Pages
- 247-252
- ISSN
- 1015-9770 (ISSN-L)
- 1421-9786
- OpenAlex
- W2083694819
- Cited by
- 10
- References
- 0
- Citations by year
- 2015: 1 · 2014: 1
- FWCI
- 0.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cerebrovascular Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
- Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- neurotrophic factors, focal cerebral ischemia, preconditioning, ischemic preconditioning, chemical preconditioning, nerve growth factor, brain-derived neurotrophic factor, ciliary neurotrophic factor, BDNF mRNA expression, middle cerebral artery occlusion, neuroprotection, reperfusion, cerebral cortex
- MeSH terms
- Animals, Antihypertensive Agents, Brain Ischemia, Male, Nitro Compounds, Propionates, RNA, Messenger, Gene Expression, Rats, Wistar, Ischemic Preconditioning, Brain-Derived Neurotrophic Factor, Infarction, Middle Cerebral Artery, Nerve Growth Factor, Ciliary Neurotrophic Factor, Rats
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