Inhibition of lysosomal degradation in retinal pigment epithelium cells induces exocytosis of phagocytic residual material at the basolateral plasma membrane
Abstract
PURPOSE: To analyze chloroquine-induced morphological changes in the retinal pigment epithelium (RPE) and Bruch's membrane (BM). METHODS: Retina-choroid complexes of chloroquine-treated Long-Evans rats were analyzed by electron microscopy. RESULTS: Intercellular spaces between the RPE cells and BM were enlarged. Residual material from phagosomes was released into these enlarged spaces. Debris accumulated within BM and encircled choriocapillaris endothelial cells. CONCLUSION: There is a release of undegraded phagocytic material (rod outer segments) into the extracellular space between BM and RPE cells, following inhibition of lysosomal degradation. Electron-dense deposits in BM and choriocapillaris may lead to reduced oxygen and nutrition flow.
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Defects in lysosomal acidification through chronic administration of lysosomal alkalinizing agents result in extrusion of undigested OS phagocytic material from the basal RPE into the space between Bruch's membrane and the RPE.
“Defects in lysosomal acidification through chronic administration of lysosomal alkalinizing agents result in extrusion of undigested OS phagocytic material from the basal RPE into the space between Bruch’s membrane and the RPE (Peters et al., 2006).”
From The cell biology of the retinal pigment epithelium (2020) · cites this paper as Peters et al., 2006
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References 19 · Cited by 41
- Authors
- Peters, S., Reinthal, E., Blitgen-Heinecke, P., Bartz-Schmidt, K.-U., & Schraermeyer, U.
- Affiliations
- Universitätsklinikum Tübingen (DE)
- University of Tübingen (DE)
- ORCID
- Schraermeyer, Ulrich
- Venue
- Ophthalmic Research
- Publisher
- S. Karger AG
- Published
- 2006
- Volume
- 38
- Issue
- 2
- Pages
- 83-88
- ISSN
- 0030-3747 (ISSN-L)
- 1423-0259
- OpenAlex
- W1992874527
- Cited by
- 41
- References
- 19
- Citations by year
- 2026: 1 · 2024: 3 · 2023: 1 · 2021: 1 · 2020: 4 · 2019: 2 · 2018: 6 · 2017: 2 · 2015: 3 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 1.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Ophthalmic Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Ophthalmology › Retinal Diseases and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinal Development and Disorders
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Keywords
- retinal pigment epithelium, chloroquine, phagocytosis, exocytosis, Bruch's membrane, rod outer segments, electron-dense deposits, choroid
- MeSH terms
- Animals, Antimalarials, Cell Membrane, Chloroquine, Exocytosis, Lysosomes, Microscopy, Electron, Phagocytosis, Pigment Epithelium of Eye, Bruch Membrane, Rats, Long-Evans, Rats
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