Effect of 5α-Dihydrotestosterone and Testosterone on Apoptosis in Human Dermal Papilla Cells
Abstract
Pathogenetic mechanisms in androgenetic alopecia are not yet fully understood; however, it is commonly accepted that androgens like testosterone (T) and 5alpha-dihydrotestosterone (5alpha-DHT) inhibit hair follicle activity with early induction of the catagen. Thus, we investigated the influence of T and 5alpha-DHT on proliferation, cell death and bcl-2/bax expression in cultured dermal papilla cells (DPC) from nonbalding scalp regions of healthy volunteers. T and 5alpha-DHT induced apoptosis in DPC in a dose-dependent and time-related manner; in addition a necrotic effect due to T at 10(-5) M was found. Interestingly, bcl-2 protein expression was decreased in T- and 5alpha-DHT-treated cells, leading to an increase in the bax/bcl-2 ratio. In addition, T and 5alpha-DHT induced proteolytic cleavage of caspase 8 and inhibited proliferation of DPC at 10(-5) M. High concentrations of T and 5alpha-DHT were needed to induce apoptotic effects in DPC. These data suggest that DPC from nonbalding scalp regions do have the capacity to undergo apoptosis, but need a high androgen stimulus. The present study provides an interesting new pathogenetic approach in androgenetic alopecia.
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Very high doses of testosterone or DHT induce apoptosis of dermal papilla cells through the bcl-2 pathway.
“Very high doses of T or DHT induce apoptosis of DPCs through the bcl-2 pathway [193].”
From Steroidogenesis in the skin: Implications for local immune functions (2013) · cites this paper as [193]
Androgen receptor co-activator expression is higher in dermal papilla cells from beard and frontal scalp than from occipital scalp.
“and the expression of AR co-activator was higher in DPCs localized at the beard and frontal scalp than in the occipital scalp [192].”
From Steroidogenesis in the skin: Implications for local immune functions (2013) · cites this paper as [192]
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References 56 · Cited by 82
- Authors
- Winiarska, A., Mandt, N., Kamp, H., Hossini, A., Seltmann, H., Zouboulis, C. C., & Blume-Peytavi, U.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- Venue
- Skin Pharmacology and Physiology
- Publisher
- S. Karger AG
- Published
- 2006
- Volume
- 19
- Issue
- 6
- Pages
- 311-321
- ISSN
- 1422-2868 (ISSN-L)
- 1660-5527
- 1660-5535
- OpenAlex
- W2062680123
- Cited by
- 82
- References
- 56
- Citations by year
- 2026: 8 · 2025: 11 · 2024: 5 · 2023: 5 · 2022: 6 · 2021: 7 · 2020: 3 · 2019: 3 · 2018: 1 · 2017: 4 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 1
- FWCI
- 2.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Skin Pharmacology and Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Urology › Hair Growth and Disorders
- Health Sciences › Medicine › Reproductive Medicine › Sperm and Testicular Function
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Prostate Cancer Treatment and Research
- Keywords
- dihydrotestosterone, testosterone, dermal papilla cells, apoptosis, androgenetic alopecia, Bcl-2, Bax, caspase-8, cell proliferation, necrosis, hair follicle, catagen
- MeSH terms
- Cell Survival, Humans, Necrosis, RNA, Skin, Dihydrotestosterone, Testosterone, Tetrazolium Salts, Thiazoles, Blotting, Western, Apoptosis, Genes, bcl-2, Reverse Transcriptase Polymerase Chain Reaction, bcl-2-Associated X Protein, Caspase 3, Caspase 8, In Vitro Techniques
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