Caffeine inhibits migration in glioma cells through the ROCK-FAK pathway
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Aims Glioma is the most malignant brain tumor that has the ability to migrate and invade the CNS. In this study, we investigated the signaling mechanism of caffeine on the migration of glioma cells. Methods The effect of caffeine on cell migration was evaluated using Transwell and wound healing assays. The expression of the focal adhesion complex as it related to cell migration was assayed using Western blotting and immunostaining. Results Caffeine decreased the migration of rat C6 and human U87MG glioma cells and down-regulated the expression of phosphorylated focal adhesion kinase (p-FAK) and p-paxillin. Caffeine also decreased p-FAK staining at the edge of glioma cells and disassembled actin stress fibers. Additionally, caffeine elevated expression of phosphorylated myosin light chain (p-MLC), an effect that could be blocked by Y27632, a rho-associated protein kinase (ROCK) inhibitor, but not myosin light chain kinase inhibitor, ML-7. Y27632 also inhibited the caffeine-reduced expression of p-FAK and p-paxillin as well as cell migration. Conclusion Caffeine decreased the migration of glioma cell through the ROCK-focal adhesion complex pathway; this mechanism may be useful as part of clinical therapy in the future.
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The normal F-Actin cytoskeleton is distributed cortically and is localized in stress fibers and focal adhesions that attach the cell surface to the substrate.
“We observed, in C6-glial cells, that the normal F-Actin cytoskeleton is distributed cortically and is localized in stress fibers and focal adhesions that attach the cell surface to the substrate [57].”
From Expression of Tau Produces Aberrant Plasma Membrane Blebbing in Glial Cells Through RhoA-ROCK-Dependent F-Actin Remodeling (2016) · cites this paper as [57]
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Update on caffeine consumption, disposition and action
Mandel, H. G. (2002) · Food and Chemical Toxicology · doi:10.1016/s0278-6915(02)00093-5 · Cited by 240 · see citing works
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Torres-Cruz, F. M., Rodríguez-Cruz, F., Escobar-Herrera, J., Barragán-Andrade, N., Basurto-Islas, G., Ripova, D., Ávila, J., & Garcia-Sierra, F. (2016) · Journal of Alzheimer's Disease · doi:10.3233/jad-150396 · Cited by 19 · see citing works
- Authors
- Chen, Y., Chou, W.-C., Ding, Y.-M., & Wu, Y.-C.
- Affiliations
- National Defense Medical Center (TW)
- Ching Kuo Institute of Management and Health (TW)
- ORCID
- Chen, Ying
- Venue
- Cellular Physiology and Biochemistry
- Publisher
- S. Karger AG
- Published
- 2014
- Volume
- 33
- Issue
- 6
- Pages
- 1888-1898
- ISSN
- 1015-8987 (ISSN-L)
- 1421-9778
- OpenAlex
- W1965776560
- Cited by
- 30
- References
- 1
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2021: 7 · 2020: 1 · 2019: 2 · 2018: 3 · 2017: 3 · 2016: 5 · 2015: 3
- FWCI
- 1.69
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cellular Physiology and Biochemistry (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Keywords
- caffeine, glioma, cell migration, rocks, focal adhesion kinase, focal adhesions, paxillin, myosin light chain phosphorylation, stress fibers, Y-27632, glioma invasion
- MeSH terms
- Amides, Central Nervous System Stimulants, Animals, Caffeine, Cell Adhesion, Cell Movement, Cell Survival, Dose-Response Relationship, Drug, Enzyme Inhibitors, Glioma, Humans, Microscopy, Fluorescence, Phosphorylation, Pyridines, Time Factors, Blotting, Western, Signal Transduction, Myosin Light Chains, Cell Line, Tumor, Rats, Focal Adhesion Protein-Tyrosine Kinases, Paxillin, rho-Associated Kinases
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