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Cited by 301

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Brain Natriuretic Peptide Is Produced in Cardiac Fibroblasts and Induces Matrix Metalloproteinases

Tsuruda, Toshihiro, Boerrigter, Guido, Huntley, Brenda K., Noser, Josh A., Cataliotti, Alessandro, Costello-Boerrigter, Lisa C., Chen, Horng H., Burnett, John C.·2002·Circulation Research
DOI 10.1161/01.res.0000046234.73401.70
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Abstract

Cardiac fibroblasts (CFs) produce extracellular matrix proteins and participate in the remodeling of the heart. It is unknown if brain natriuretic peptide (BNP) is synthesized by CFs and if BNP participates in the regulation of extracellular matrix turnover. In this study, we examined the production of BNP in adult canine CFs and the role of BNP and its signaling system on collagen synthesis and on the activation of matrix metalloproteinases (MMPs). BNP mRNA was detected in CFs, and a specific radioimmunoassay demonstrated that BNP 1-32 was secreted into the media at a rate of 11.2±1.0 pg/10 5 cells per 48 hours (mean±SEM). The amount of BNP secretion was significantly ( P <0.01) augmented by 10 −7 mol/L tumor necrosis factor-α in a time-dependent manner. BNP significantly ( P <0.01) inhibited de novo collagen synthesis as assessed by [ 3 H]proline incorporation, whereas zymographic MMP-2 (gelatinase) abundance was significantly ( P <0.05) stimulated by BNP between 10 −7 and 10 −6 mol/L. In addition, protein expression of MMP-1, -2, and -3 and membranous type-1 MMP was significantly increased by 10 −6 mol/L BNP. The cGMP analogue 8-bromo-cGMP (10 −4 mol/L) mimicked the BNP effect, whereas inhibition of protein kinase G by KT5823 (10 −6 mol/L) significantly ( P <0.05) attenuated BNP-induced zymographic MMP-2 abundance. In summary, this study reports that BNP is present in cultured CFs and that BNP decreases collagen synthesis and increases MMPs via cGMP–protein kinase G signaling. These in vitro findings support a role for BNP as a regulator of myocardial structure via control of cardiac fibroblast function.

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Metadata

Authors
Tsuruda, T., Boerrigter, G., Huntley, B. K., Noser, J. A., Cataliotti, A., Costello-Boerrigter, L. C., Chen, H. H., & Burnett, J. C.
Venue
Circulation Research
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2002-12-13
Volume
91
Issue
12
Pages
1127-1134
ISSN
  • 0009-7330 (ISSN-L)
  • 1524-4571
DOI
10.1161/01.res.0000046234.73401.70
OpenAlex
W2136718447
Cited by
301
References
37
FWCI
11.63
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Brain Natriuretic Peptide Is Produced in Cardiac Fibroblasts and Induces Matrix Metalloproteinases. https://app.cochat.ai/papers/10.1161/01.res.0000046234.73401.70
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Tsuruda, T., Boerrigter, G., Huntley, B. K., Noser, J. A., Cataliotti, A., Costello-Boerrigter, L. C., Chen, H. H., & Burnett, J. C. (2002). Brain Natriuretic Peptide Is Produced in Cardiac Fibroblasts and Induces Matrix Metalloproteinases. Circulation Research. https://doi.org/10.1161/01.res.0000046234.73401.70

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