The DOI matches its Crossref record; 58 of the 58 references it cites were found in a registry or an index.
Nitric Oxide Scavenging by Red Blood Cell Microparticles and Cell-Free Hemoglobin as a Mechanism for the Red Cell Storage Lesion
Open accessAbstract
Background—: Intravascular red cell hemolysis impairs nitric oxide (NO)–redox homeostasis, producing endothelial dysfunction, platelet activation, and vasculopathy. Red blood cell storage under standard conditions results in reduced integrity of the erythrocyte membrane, with formation of exocytic microvesicles or microparticles and hemolysis, which we hypothesized could impair vascular function and contribute to the putative storage lesion of banked blood. Methods and Results—: We now find that storage of human red blood cells under standard blood banking conditions results in the accumulation of cell-free and microparticle-encapsulated hemoglobin, which, despite 39 days of storage, remains in the reduced ferrous oxyhemoglobin redox state and stoichiometrically reacts with and scavenges the vasodilator NO. Using stopped-flow spectroscopy and laser-triggered NO release from a caged NO compound, we found that both free hemoglobin and microparticles react with NO about 1000 times faster than with intact erythrocytes. In complementary in vivo studies, we show that hemoglobin, even at concentrations below 10 μmol/L (in heme), produces potent vasoconstriction when infused into the rat circulation, whereas controlled infusions of methemoglobin and cyanomethemoglobin, which do not consume NO, have substantially reduced vasoconstrictor effects. Infusion of the plasma from stored human red blood cell units into the rat circulation produces significant vasoconstriction related to the magnitude of storage-related hemolysis. Conclusions—: The results of these studies suggest new mechanisms for endothelial injury and impaired vascular function associated with the most fundamental of storage lesions, hemolysis.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Anemia and Blood Transfusion in Critically Ill Patients
Vincent, J. L. (2002) · JAMA · doi:10.1001/jama.288.12.1499 · Cited by 1,821
Rother, R. P., Bell, L., Hillmen, P., & Gladwin, M. T. (2005) · JAMA · doi:10.1001/jama.293.13.1653 · Cited by 1,557
Self-citationDuration of Red-Cell Storage and Complications after Cardiac Surgery
Koch, C. G., Li, L., Sessler, D. I., Figueroa, P., Hoeltge, G. A., Mihaljevic, T., & Blackstone, E. H. (2008) · New England Journal of Medicine · doi:10.1056/nejmoa070403 · Cited by 1,363
Corwin, H. L., Gettinger, A., Pearl, R. G., Fink, M. P., Levy, M. M., Abraham, E., MacIntyre, N. R., Shabot, M. M., Duh, M.-S., & Shapiro, M. J. (2004) · Critical Care Medicine · doi:10.1097/01.ccm.0000104112.34142.79 · Cited by 1,348
Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
Reiter, C. D., Wang, X., Tanus-Santos, J. E., Hogg, N., Cannon, R. O., Schechter, A. N., & Gladwin, M. T. (2002) · Nature Medicine · doi:10.1038/nm1202-799 · Cited by 1,330
Self-citationPulmonary Hypertension as a Risk Factor for Death in Patients with Sickle Cell Disease
Gladwin, M. T., Sachdev, V., Jison, M. L., Shizukuda, Y., Plehn, J. F., Minter, K., Brown, B., Coles, W. A., Nichols, J. S., Ernst, I., et al. (2004) · New England Journal of Medicine · doi:10.1056/nejmoa035477 · Cited by 1,325
Self-citationAdult Respiratory Distress Syndrome: Risk with Common Predispositions
FOWLER, A. A., HAMMAN, R. F., GOOD, J. T., BENSON, K. N., BAIRD, M., EBERLE, D. J., PETTY, T. L., & HYERS, T. M. (1983) · Annals of Internal Medicine · doi:10.7326/0003-4819-98-5-593 · Cited by 794
Morris, C. R. (2005) · JAMA · doi:10.1001/jama.294.1.81 · Cited by 763
Simulation of the diffusion and reaction of endogenously produced nitric oxide.
Lancaster, J. R. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.17.8137 · Cited by 698
Kato, G. J., McGowan, V., Machado, R. F., Little, J. A., Taylor, J., Morris, C. R., Nichols, J. S., Wang, X., Poljakovic, M., Morris, S. M., et al. (2006) · Blood · doi:10.1182/blood-2005-06-2373 · Cited by 661
Self-citation
Cited by
Schaer, D. J., Buehler, P. W., Alayash, A. I., Belcher, J. D., & Vercellotti, G. M. (2013) · Blood · doi:10.1182/blood-2012-11-451229 · Cited by 754
Intravascular hemolysis and the pathophysiology of sickle cell disease
Kato, G. J., Steinberg, M. H., & Gladwin, M. T. (2017) · Journal of Clinical Investigation · doi:10.1172/jci89741 · Cited by 748
Self-citationFergusson, D. A., Hébert, P., Hogan, D. L., LeBel, L., Rouvinez-Bouali, N., Smyth, J. A., Sankaran, K., Tinmouth, A., Blajchman, M. A., Kovacs, L., et al. (2012) · JAMA · doi:10.1001/2012.jama.11953 · Cited by 440
Microvesicles as Cell–Cell Messengers in Cardiovascular Diseases
Loyer, X., Vion, A.-C., Tedgui, A., & Boulanger, C. M. (2014) · Circulation Research · doi:10.1161/circresaha.113.300858 · Cited by 408
Anemia in Critical Illness: Insights into Etiology, Consequences, and Management
Hayden, S. J., Albert, T. J., Watkins, T. R., & Swenson, E. R. (2012) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/rccm.201110-1915ci · Cited by 271
Metadata
- Authors
- Donadee, C., Raat, N. J. H., Kanias, T., Tejero, J., Lee, J. S., Kelley, E. E., Zhao, X., Liu, C., Reynolds, H., Azarov, I., Frizzell, S., Meyer, E. M., Donnenberg, A. D., Qu, L., Triulzi, D., Kim-Shapiro, D. B., & Gladwin, M. T.
- Venue
- Circulation
- Published
- 2011-07-26
- Volume
- 124
- Issue
- 4
- Pages
- 465-476
- ISSN
- 0009-7322 (ISSN-L)
- 1524-4539
- OpenAlex
- W2122036831
- Cited by
- 572
- References
- 58
- FWCI
- 28.19
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Nitric Oxide Scavenging by Red Blood Cell Microparticles and Cell-Free Hemoglobin as a Mechanism for the Red Cell Storage Lesion. https://app.cochat.ai/papers/10.1161/circulationaha.110.008698
- The paper
- Donadee, C., Raat, N. J. H., Kanias, T., Tejero, J., Lee, J. S., Kelley, E. E., Zhao, X., Liu, C., Reynolds, H., Azarov, I., Frizzell, S., Meyer, E. M., Donnenberg, A. D., Qu, L., Triulzi, D., Kim-Shapiro, D. B., & Gladwin, M. T. (2011). Nitric Oxide Scavenging by Red Blood Cell Microparticles and Cell-Free Hemoglobin as a Mechanism for the Red Cell Storage Lesion. Circulation. https://doi.org/10.1161/circulationaha.110.008698
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
