Comparison of methods: passing and Bablok regression

Abstract

The com pa ri son of met ho ds expe ri me nt is im por ta nt pa rt in pro ce ss of ana lyti cal met ho ds and in stru men ts va li da tion.Pas si ng and Bab lok reg ression ana lysis is a sta tis ti cal pro ce du re that al lows va luab le es ti ma tion of ana lyti cal met ho ds ag ree me nt and pos sib le syste ma tic bias be tween them.It is ro bu st, no n-pa ra met ric, non sen si ti ve to dis tri bu tion of errors and da ta out lie rs.As sum ptio ns for pro per ap pli ca tion of Pas si ng and Bab lok regres sion are con ti nuous ly dis tri bu ted da ta and li near re la tion ship be tween da ta mea su red by two ana lyti cal met ho ds.Re sul ts are pre sen ted wi th scat ter diag ram and reg res sion li ne, and reg res sion equa tion whe re in ter ce pt rep re sen ts con sta nt and slo pe pro por tio nal mea su re me nt er ror.Confi den ce in ter va ls of 95% of in ter ce pt and slo pe explain if their va lue diff er from va lue ze ro (in ter ce pt) and va lue one (slo pe) on ly by chan ce, al lowi ng con clu sion of met hod ag ree me nt and cor rec tion ac tion if ne ces sa ry.Re si dual plot re vea led out lie rs and iden ti fy pos sib le no n-li nea ri ty.Fur ther mo re, cu mu la ti ve sum li nea ri ty te st is per for med to in ves ti ga te pos sib le sig ni fi ca nt de via tion from li nea ri ty be tween two sets of da ta.Non li near sam ples are not sui tab le for con clu di ng on met hod ag ree me nt.Key wor ds: com pa ri son of met ho ds; data ana lysis; Pas si ng and Bab lok reg res sion Re cei ved: Janua ry 6, 2011 Ac cep ted: Janua ry 16, 2011 Com pa ri son of met ho ds: Pas si ng and Bab lok reg res sion Li di ja Bi li ć-Zul le Cli ni cal De par tme nt of La bo ra to ry Diag nos ti cs, Cli ni cal Hos pi tal Cen tre and De par tme nt of Me di cal In for ma ti cs, Ri je ka Uni ver si ty School of Me di ci ne, Ri je ka, Croa tia Cor res pon di ng aut hor: li di ja.bilic-zulle@medri.hr