The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
IL-4 and IL-5 mRNA and protein in bronchial biopsies from patients with atopic and nonatopic asthma: evidence against "intrinsic" asthma being a distinct immunopathologic entity.
Abstract
Intrinsic (nonatopic) asthma is considered to be a distinct pathogenetic variant of asthma since, unlike extrinsic (atopic) asthma, patients with the disease are skin test-negative to common aeroallergens, and have total serum IgE concentrations within the normal range. Nevertheless, the recent demonstration of increased numbers of cells expressing the high-affinity IgE receptor in bronchial biopsies from atopic and nonatopic asthmatic subjects, together with epidemiologic evidence indicating that serum IgE concentrations relate closely to asthma prevalence regardless of atopic status, suggests that IgE-mediated mechanisms may participate in the pathogenesis of both atopic and nonatopic asthma. Furthermore both variants of the disease are associated with bronchial mucosal eosinophilic inflammation. Interleukin-4 (IL-4) is an essential cofactor for IgE synthesis, and there is strong evidence that IL-5 plays a major role in eosinophil accumulation in asthmatic inflammation. For these reasons we compared the expression of IL-4 and IL-5 mRNA and protein product using a semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) amplification, in situ hybridization, and immunohistochemistry in bronchial biopsies from symptomatic atopic and nonatopic asthmatic subjects and atopic and nonatopic controls. The results showed that as compared with controls, biopsies from both groups of asthmatic subjects had increased numbers of IL-4 and IL-5 mRNA copies relative to beta-actin mRNA as detected by RT-PCR. Similarly, in situ hybridization and immunohistochemistry demonstrated increased numbers of cells expressing IL-4 and IL-5 mRNA and protein in asthmatic subjects, irrespective of their atopic status. We conclude that individuals with either atopic or nonatopic asthma show infiltration of the bronchial mucosa with cells expressing Th2-type cytokines, providing further evidence for similarities in the immunopathogenesis of these clinically distinct forms of asthma.
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Cites
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90021-2 · Cited by 46,426
Eosinophilic Inflammation in Asthma
Bousquet, J., Chanez, P., Lacoste, J. Y., Barnéon, G., Ghavanian, N., Enander, I., Venge, P., Ahlstedt, S., Simony-Lafontaine, J., Godard, P., et al. (1990) · New England Journal of Medicine · doi:10.1056/nejm199010113231505 · Cited by 2,499
Association of Asthma with Serum IgE Levels and Skin-Test Reactivity to Allergens
Burrows, B., Martinez, F. D., Halonen, M., Barbee, R. A., & Cline, M. G. (1989) · New England Journal of Medicine · doi:10.1056/nejm198902023200502 · Cited by 1,659
Recombinant human interleukin 5 is a selective activator of human eosinophil function.
Lopez, A. F., Sanderson, C. J., Gamble, J. R., Campbell, H. D., Young, I. G., & Vadas, M. A. (1988) · The Journal of experimental medicine · doi:10.1084/jem.167.1.219 · Cited by 951
Expression of mRNA for interleukin-5 in mucosal bronchial biopsies from asthma.
Hamid, Q., Azzawi, M., Ying, S., Moqbel, R., Wardlaw, A. J., Corrigan, C. J., Bradley, B., Durham, S. R., Collins, J. V., & Jeffery, P. K. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115166 · Cited by 833
Self-citationBradding, P., Roberts, J. A., Britten, K. M., Montefort, S., Djukanovic, R., Mueller, R., Heusser, C. H., Howarth, P. H., & Holgate, S. T. (1994) · American Journal of Respiratory Cell and Molecular Biology · doi:10.1165/ajrcmb.10.5.8179909 · Cited by 830
Walker, C., Bode, E., Boer, L., Hansel, T. T., Blaser, K., & Virchow, J.-C. (1992) · American Review of Respiratory Disease · doi:10.1164/ajrccm/146.1.109 · Cited by 768
Del Prete, G., Maggi, E., Parronchi, P., Chrétien, I., Tiri, A., Macchia, D., Ricci, M., Banchereau, J., De Vries, J., & Romagnani, S. (1988) · The Journal of Immunology · doi:10.4049/jimmunol.140.12.4193 · Cited by 707
Cartier, A., Thomson, N. C., Frith, P. A., Roberts, R., & Hargreave, F. E. (1982) · Journal of Allergy and Clinical Immunology · doi:10.1016/0091-6749(82)90038-0 · Cited by 617
T cells and eosinophils in the pathogenesis of asthma
Corrigan, C. J., & Kay, A. B. (1992) · Immunology Today · doi:10.1016/0167-5699(92)90026-4 · Cited by 570
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Asthma: From Bronchoconstriction to Airways Inflammation and Remodeling
BOUSQUET, J., JEFFERY, P. K., BUSSE, W. W., JOHNSON, M., & VIGNOLA, A. M. (2000) · American Journal of Respiratory and Critical Care Medicine · doi:10.1164/ajrccm.161.5.9903102 · Cited by 1,702
Lambrecht, B. N., & Hammad, H. (2015) · Nature Immunology · doi:10.1038/ni.3049 · Cited by 1,673
Distinguishing severe asthma phenotypes
Miranda, C., Busacker, A., Balzar, S., Trudeau, J., & Wenzel, S. E. (2004) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2003.10.041 · Cited by 586
Allergic and nonallergic forms of atopic diseases
Novak, N., & Bieber, T. (2003) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2003.1595 · Cited by 441
Robinson, D., Humbert, M., Buhl, R., Cruz, A. A., Inoue, H., Korom, S., Hanania, N. A., & Nair, P. (2017) · Clinical & Experimental Allergy · doi:10.1111/cea.12880 · Cited by 357
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Metadata
- Authors
- Humbert, M., Durham, S. R., Ying, S., Kimmitt, P., Barkans, J., Assoufi, B., Pfister, R., Menz, G., Robinson, D. S., Kay, A. B., & Corrigan, C. J.
- Venue
- American Journal of Respiratory and Critical Care Medicine
- Publisher
- Oxford University Press (OUP)
- Published
- 1996-11
- Volume
- 154
- Issue
- 5
- Pages
- 1497-1504
- ISSN
- 1073-449X (ISSN-L)
- 1535-4970
- OpenAlex
- W2021451119
- Cited by
- 406
- References
- 18
- FWCI
- 15.15
- Type
- journal article
- Language
- en
- Open access
- closed
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- Humbert, M., Durham, S. R., Ying, S., Kimmitt, P., Barkans, J., Assoufi, B., Pfister, R., Menz, G., Robinson, D. S., Kay, A. B., & Corrigan, C. J. (1996). IL-4 and IL-5 mRNA and protein in bronchial biopsies from patients with atopic and nonatopic asthma: evidence against "intrinsic" asthma being a distinct immunopathologic entity. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/ajrccm.154.5.8912771
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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