The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Cigarette Smoke Induces Senescence in Alveolar Epithelial Cells
Abstract
Cellular senescence is a state of irreversible growth arrest induced either by telomere shortening (replicative senescence) or by telomere-independent signals (stress-induced senescence). The alveolar epithelium is often injured by a variety of inhaled toxins, including cigarette smoke (CS). In the present study, we investigated whether exposure to CS induces senescence of alveolar epithelial cells. In vitro experiments showed that exposure of A549 cells or normal human alveolar epithelial cells to sublethal concentrations of aqueous CS extracts induced cellular senescence. The senescence was characterized by a dose- and time-dependent increase in senescence-associated beta-galactosidase activity, senescence-associated changes in cell morphology, an increase in cell size and lysosomal mass, accumulation of lipofuscin, overexpression of p21(CIP1/WAF1/Sdi1) protein, and irreversible growth arrest. In vivo experiments in Institute for Cancer Research mice showed that inhalation of CS for 2 wk induced increases in senescence-associated beta-galactosidase activity, lipofuscin accumulation, and p21(CIP1/WAF1/Sdi1) protein expression in alveolar epithelial cells. These results suggest that CS induces a phenotype that is indistinguishable from that of senescence in alveolar epithelial cells. The induction of cellular senescence by CS may contribute to impaired re-epithelialization, leading to CS-related chronic lung diseases.
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Cites
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Cited by
Cellular senescence: from physiology to pathology
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Alveolar Cell Senescence in Patients with Pulmonary Emphysema
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Self-citationRedox regulation of SIRT1 in inflammation and cellular senescence
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SIRT1 protects against emphysema via FOXO3-mediated reduction of premature senescence in mice
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Metadata
- Authors
- Tsuji, T., Aoshiba, K., & Nagai, A.
- Venue
- American Journal of Respiratory Cell and Molecular Biology
- Publisher
- Oxford University Press (OUP)
- Published
- 2004-12
- Volume
- 31
- Issue
- 6
- Pages
- 643-649
- ISSN
- 1044-1549 (ISSN-L)
- 1535-4989
- OpenAlex
- W1989966522
- Cited by
- 241
- References
- 7
- FWCI
- 3.80
- Type
- journal article
- Language
- en
- Open access
- closed
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- Tsuji, T., Aoshiba, K., & Nagai, A. (2004). Cigarette Smoke Induces Senescence in Alveolar Epithelial Cells. American Journal of Respiratory Cell and Molecular Biology. https://doi.org/10.1165/rcmb.2003-0290oc
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