The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Chitosan Nanoparticles as a Potential Drug Delivery System for the Ocular Surface: Toxicity, Uptake Mechanism and In Vivo Tolerance
Abstract
PURPOSE: To study the in vitro and in vivo interaction of chitosan nanoparticles (CSNPs), a new particulate drug carrier, with epithelial cells on the ocular surface. METHODS: CSNPs labeled with fluorescein isothiocyanate-bovine serum albumin were produced by ionotropic gelation. Human conjunctival epithelial cells (IOBA-NHC) were exposed for 15, 30, 60, and 120 minutes to three different CSNP concentrations. Immediately after treatment and after a 24-hour recovery period in culture medium, cell survival, and viability were measured. The association of CSNPs with IOBA-NHC cells was investigated by confocal microscopy. The influence of temperature and the effect of metabolic inhibition were studied by fluorometry. The in vivo uptake and acute tolerance of the ocular surface to CSNPs were evaluated in rabbits. RESULTS: Cell survival and viability of CSNP-exposed cells were equivalent to that of the control. Uptake of CSNPs was continuous for the 2-hour duration of these experiments and was temperature dependent. Metabolic inhibition by sodium azide had no effect on CSNP uptake. The rabbit ocular surface showed no signs of inflammation or alteration after CSNP exposure compared with the control. Fluorescence microscopy of rabbit eyeball and lid sections confirmed in vivo uptake by conjunctival and corneal epithelia. CONCLUSIONS: CSNPs were internalized by IOBA-NHC cells by an active transport mechanism that did not compromise cell viability. Moreover, these nanoparticles were well tolerated by the ocular surface tissues. These facts add further support for the potential use of these colloidal systems to delivery drugs to the ocular surface.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers
Calvo, P., Remu��n-L�pez, C., Vila-Jato, J. L., & Alonso, M. J. (1997) · Journal of Applied Polymer Science · doi:10.1002/(sici)1097-4628(19970103)63:1<125::aid-app13>3.0.co;2-4 · Cited by 1,856
Self-citationIn vitro evaluation of mucoadhesive properties of chitosan and some other natural polymers
Lehr, C.-M., Bouwstra, J. A., Schacht, E. H., & Junginger, H. E. (1992) · International Journal of Pharmaceutics · doi:10.1016/0378-5173(92)90353-4 · Cited by 1,035
Gastrointestinal Uptake of Biodegradable Microparticles: Effect of Particle Size
Desai, M. P., Labhasetwar, V., Amidon, G. L., & Levy, R. J. (1996) · Pharmaceutical Research · doi:10.1023/a:1016085108889 · Cited by 941
Nanoparticle Uptake by the Rat Gastrointestinal Mucosa: Quantitation and Particle Size Dependency
Jani, P., Halbert, G. W., Langridge, J., & Florence, A. T. (1990) · Journal of Pharmacy and Pharmacology · doi:10.1111/j.2042-7158.1990.tb07033.x · Cited by 920
Chitosan: A Unique Polysaccharide for Drug Delivery
Felt, O., Buri, P., & Gurny, R. (1998) · Drug Development and Industrial Pharmacy · doi:10.3109/03639049809089942 · Cited by 769
De Campos, A. M., Sánchez, A., & Alonso, M. J. (2001) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(01)00760-8 · Cited by 666
Self-citationDesign of biodegradable particles for protein delivery
Vila, A., Sánchez, A., Tobı́o, M., Calvo, P., & Alonso, M. J. (2002) · Journal of Controlled Release · doi:10.1016/s0168-3659(01)00486-2 · Cited by 620
Self-citationEnhancement of Nasal Absorption of Insulin Using Chitosan Nanoparticles
Fernández-Urrusuno, R., Calvo, P., Remuñán-López, C., Vila-Jato, J. L., & José Alonso, M. (1999) · Pharmaceutical Research · doi:10.1023/a:1018908705446 · Cited by 587
A practical note on the use of cytotoxicity assays
Weyermann, J., Lochmann, D., & Zimmer, A. (2005) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2004.09.018 · Cited by 525
Low molecular weight chitosan nanoparticles as new carriers for nasal vaccine delivery in mice
Vila, A., Sánchez, A., Janes, K., Behrens, I., Kissel, T., Jato, J. L. V., & Alonso, M. J. (2004) · European Journal of Pharmaceutics and Biopharmaceutics · doi:10.1016/j.ejpb.2003.09.006 · Cited by 449
Self-citationde Campos, A. M., Diebold, Y., Carvalho, E. L. S., Sánchez, A., & José Alonso, M. (2004) · Pharmaceutical Research · doi:10.1023/b:pham.0000026432.75781.cb · Cited by 404
Self-citationBehrens, I., Pena, A. I. V., Alonso, M. J., & Kissel, T. (2002) · Pharmaceutical Research · doi:10.1023/a:1019854327540 · Cited by 372
Self-citationThe potential of chitosan in ocular drug delivery
Alonso, M. J., & Sánchez, A. (2003) · Journal of Pharmacy and Pharmacology · doi:10.1211/0022357022476 · Cited by 352
Self-citationProgress in Biomedical Polymers
(1990) · Springer eBooks · doi:10.1007/978-1-4899-0768-4 · Cited by 250
De Campos, A. M., Sánchez, A., Gref, R., Calvo, P., & Alonso, M. J. (2003) · European Journal of Pharmaceutical Sciences · doi:10.1016/s0928-0987(03)00178-7 · Cited by 234
Self-citationChitosan: A Biocompatible Material for Oral and Intravenous Administrations
Hirano, S., Seino, H., Akiyama, Y., & Nonaka, I. (1990) · Progress in Biomedical Polymers · doi:10.1007/978-1-4899-0768-4_28 · Cited by 219
Epithelial Mucins of the Ocular Surface: Structure, Biosynthesis and Function
Argüeso, P., & Gipson, I. K. (2001) · Experimental Eye Research · doi:10.1006/exer.2001.1045 · Cited by 215
Microparticulate Systems for the Delivery of Proteins and Vaccines
(2020) · CRC Press eBooks · doi:10.1201/9781003067542 · Cited by 199
Impression cytology of the ocular surface: a review
Calonge, M., Diebold, Y., Sáez, V., de Salamanca, A. E., Garcı́a-Vázquez, C., Corrales, R. M., & Herreras, J. M. (2004) · Experimental Eye Research · doi:10.1016/j.exer.2003.09.009 · Cited by 182
Self-citationLosa, C., Marchal-Heussler, L., Orallo, F., Jato, J. L. V., & Alonso, M. J. (1993) · Pharmaceutical Research · doi:10.1023/a:1018977130559 · Cited by 163
Self-citationCharacterization of a Spontaneously Immortalized Cell Line (IOBA-NHC) from Normal Human Conjunctiva
Diebold, Y., Calonge, M., Enríquez‐de‐Salamanca, A., Callejo, S., Corrales, R. M., ́ez, V. S., Siemasko, K. F., & Stern, M. E. (2003) · Investigative Ophthalmology & Visual Science · doi:10.1167/iovs.03-0560 · Cited by 150
Self-citationPolyester Nanocapsules as New Topical Ocular Delivery Systems for Cyclosporin A
Calvo, P., Sánchez, A., Martínez, J., López, M. I., Calonge, M., Jimeno, J. C. P., & Alonso, M. J. (1996) · Pharmaceutical Research · doi:10.1023/a:1016015803611 · Cited by 108
Self-citationVitamin B12-mediated transport of nanoparticles across Caco-2 cells
Russell-Jones, G. J. (1999) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(98)00394-9 · Cited by 106
Losa, C., Calvo, P., Castro, E., Vila-Jato, J. L., & Alonso, M. J. (1991) · Journal of Pharmacy and Pharmacology · doi:10.1111/j.2042-7158.1991.tb03534.x · Cited by 102
Self-citationCalvo, P., THOMAS, C., Alonso, M. J., Vila-Jato, J. L., & Robinson, J. R. (1994) · International Journal of Pharmaceutics · doi:10.1016/0378-5173(94)90179-1 · Cited by 91
Self-citationKawazu, K., Yamada, K., Nakamura, M., & Ota, A. (1999) · PubMed · Cited by 70
Nanoparticulate Drug Carrier Technology
Alonso, M. J. (2020) · CRC Press eBooks · doi:10.1201/9781003067542-7 · Cited by 70
Self-citationSvenson, S. (2004) · ACS symposium series · doi:10.1021/bk-2004-0879 · Cited by 48
Cited by
Polymeric nanoparticulate system: A potential approach for ocular drug delivery
Nagarwal, R. C., Kant, S., Singh, P. N., Maiti, P., & Pandit, J. K. (2009) · Journal of Controlled Release · doi:10.1016/j.jconrel.2008.12.018 · Cited by 481
Nanoparticles for drug delivery to the anterior segment of the eye
Janagam, D. R., Wu, L., & Lowe, T. L. (2017) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2017.04.001 · Cited by 341
Ocular drug delivery by liposome–chitosan nanoparticle complexes (LCS-NP)
Diebold, Y., Jarrín, M., Sáez, V., Carvalho, E. L. S., Orea, M., Calonge, M., Seijo, B., & Alonso, M. J. (2007) · Biomaterials · doi:10.1016/j.biomaterials.2006.11.028 · Cited by 273
Self-citationRecent developments in the use of nanoparticles for treatment of biofilms
Han, C., Romero, N., Fischer, S., Dookran, J., Berger, A., & Doiron, A. L. (2017) · Nanotechnology Reviews · doi:10.1515/ntrev-2016-0054 · Cited by 114
Ocular Nanoparticle Toxicity and Transfection of the Retina and Retinal Pigment Epithelium
Prow, T. W., Bhutto, I., Kim, S. Y., Grebe, R., Merges, C., McLeod, D. S., Uno, K., Mennon, M., Rodriguez, L., Leong, K., et al. (2008) · Nanomedicine: Nanotechnology, Biology and Medicine · doi:10.1016/j.nano.2008.06.003 · Cited by 111
Metadata
- Authors
- de Salamanca, A. E., Diebold, Y., Calonge, M., Garci´a-Vazquez, C., Callejo, S., Vila, A., & Alonso, M. J.
- Venue
- Investigative Opthalmology & Visual Science
- Published
- 2006-04-01
- Volume
- 47
- Issue
- 4
- Pages
- 1416
- ISSN
- 0146-0404 (ISSN-L)
- 1552-5783
- OpenAlex
- W2120554483
- Cited by
- 286
- References
- 28
- FWCI
- 7.41
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
