Induction of Lysosomal Dilatation, Arrested Autophagy, and Cell Death by Chloroquine in Cultured ARPE-19 Cells

Yoon, Young Hee; Cho, Kyung Sook; Hwang, Jung Jin; Lee, Sook-Jeong; Choi, Jeong A.; Koh, Jae-Young

doi:10.1167/iovs.10-5278

Induction of Lysosomal Dilatation, Arrested Autophagy, and Cell Death by Chloroquine in Cultured ARPE-19 Cells

Purpose To characterize and investigate the mechanism of chloroquine (CQ) retinotoxicity in human retinal pigment epithelium-derived ARPE-19 cells. Methods Cultured ARPE-19 cells were exposed to 10 to 250 μM CQ, and cell death was quantified using a lactate dehydrogenase release assay. Autophagy …