THE EFFECT OF ACTH, RENIN, ANGIOTENSIN II, AND VARIOUS PRECURSORS ON BIOSYNTHESIS OF ALDOSTERONE BY ADRENAL SLICES

Abstract

both of a considerable amount of zona glomerulosa in the inner slices and of functionally inactive fibrous cap- sule in the outer slice.The slices were minced with scissors and weighed.Specimens weighing about 500 mg were placed in 5 ml of Krebs-Ringer bicarbonate medium containing glucose in a concentration of 200 mg per 100 ml.At least 4 vessels were incubated and analyzed separately for each portion of each experiment.Thus, there were at least 4 control vessels and at least 4 with each precursor or stimulating agent studied in each experiment.The tissues were kept chilled until incubation, which was started between 3 and 5 hours after the death of the cattle.Tissues were preincubated for 1 hour at 37°C under 5 per cent CO2-95 per cent 02 on the Dubnoff metabolic shaker.The preincubation medium was removed, and 5 ml of fresh medium was used to transfer the slices to vessels to which the appropriate precursor had been added with or with- out ACTH, renin, or angiotensin II.Incubation was for 2 hours.