The DOI matches its Crossref record; 30 of the 32 references it cites were found in a registry or an index.
IS HEMOGLOBIN AN ESSENTIAL STRUCTURAL COMPONENT OF HUMAN ERYTHROCYTE MEMBRANES?*
Open accessAbstract
This investigation of human red cell membranes was undertaken in order to determine whether the presence of hemoglobin in the membrane is es- sential for maintenance of the membrane's struc- tural integrity, which has been evaluated by stud- ies of the shape, lipid content and composition, protein content, osmotic responsiveness, and certain enzymic activities of erythrocyte ghosts from which virtually all of the cellular hemoglobin had been removed.Many studies have been done with red cell "ghosts," or posthemolytic residues, because such preparations offer the possibility of analyzing certain physiological, biochemical, electron micro- scopic, and immunologic properties attributable to the membranes themselves, apart from the intracel- lular contents.Representative techniques for preparation of red cell ghosts include hemolysis in distilled water, the effects of which were stud- ied first by Bernstein and his associates (1), the freeze-thaw method described by Jorpes (2), the use of chemical lysins such as digitonin and sa- ponin (3) and the use of 0.1%o sodium chloride saturated with CO2 (4), both as described by Ponder, and the method of successive osmotic hemolysis proposed by Hillier and Hoffman (5).Recently, Danon, Nevo, and Marikovsky (6) have described a modification of the technique of Hil- lier and Hoffman which they have termed "gradual osmotic lysis."This method provides a high yield of ghosts that bear a remarkable resemblance
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“Low Hgb, or low HCT, the volume of red blood cells compared with total blood volume, can lead to a diminished supply of oxygen to the tissues, and therefore have a direct effect on endurance performance.”
From How does hormone transition in transgender women change body composition, muscle strength and haemoglobin? Systematic review with a focus on the implications for sport participation (2021) · cites this paper as [20]
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A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUES
Folch, J., Lees, M., & Stanley, G. H. S. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64849-5 · Cited by 65,189
(1962) · Postgraduate Medical Journal · doi:10.1136/pgmj.38.436.126-a · Cited by 1,462
PREPARATION OF LIPIDE EXTRACTS FROM BRAIN TISSUE
Folch, J., Ascoli, I., Lees, M. B., Meath, J. A., & Lebaron, F. N. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)55987-1 · Cited by 1,341
Post, R. L., Merritt, C. R., Kinsolving, C. R., & Albright, C. D. (1960) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)76884-7 · Cited by 1,060
An Introduction to Statistical Analysis.
Cochran, W. G., Dixon, W. J., & Massey, F. J. (1957) · Journal of the American Statistical Association · doi:10.2307/2280909 · Cited by 959
Adenosinetriphosphatase activity and the active movements of alkali metal ions
Dunham, E. T., & Glynn, I. M. (1961) · The Journal of Physiology · doi:10.1113/jphysiol.1961.sp006675 · Cited by 737
Standardizing a method for clinical hemoglobinometry.
Crosby, W. H., MUNN, J. I., & Furth, F. W. (1954) · PubMed · Cited by 513
(1957) · The Medical Journal of Australia · doi:10.5694/j.1326-5377.1957.tb57567.x · Cited by 378
Reed, C. F., Swisher, S. N., Marinetti, G. V., & Enen, E. G. (1960) · PubMed · Cited by 281
Self-citationGlucose 6-Phosphate Dehydrogenase from Human Erythrocytes
Kirkman, H. N. (1962) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)63446-0 · Cited by 231
Research works printed but not linked: 8
- Ponder, E. On properties of the red cell ghost. I. Volume and shape changes during haemolysis and "reversible of haemolysis." J. exp. Biol. 1942, 18, 257.
- Danon, D., A. Nevo, and Y. Marikovsky. Prepara- tion of erythrocyte ghosts by gradual haemolysis in hypotonic aqueous solution. Bull. Res. Coun. Israel E 1956, 6, 36.
- Carson, P. E., S. L. Schrier, and A. S. Alving. In- activation of glucose-6-phosphate dehydrogenase in human erythrocytes (abstract). J. Lab. clin. Med. 1956, 48, 794.
- Kirkman, H. N. Characterization of partially puri- fied glucose-6-phosphate dehydrogenase from nor- mal and primaquine-sensitive erythrocytes (ab- stract). Fed. Proc. 1959, 18, 261.
- Erickson, B. N., H. W. Williams, S. S. Bernstein, I. Avrin, R. L. Jones, and I. G. Macy. Lipid dis- tribution of posthemolytic residue or stroma of erythrocytes. J. biol. Chem. 1938, 122, 515.
- Ponder, E. Red cell structure and its breakdown. Protoplasmatologia: Handbuch der protoplasma- forschung. Vienna, Springer, 1955, 10, 53.
- Westerman, M. P., L. E. Pierce, and W. N. Jensen. Red cell and plasma lipid studies in sickle cell anemia (abstract). Blood 1960, 15, 422.
- Hoffman, J. F. Physiological characterization of hu- man red blood cell ghosts. J. gen. Physiol. 1958, 42, 9.
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Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane
Fairbanks, G., Steck, T. L., & Wallach, D. F. H. (1971) · Biochemistry · doi:10.1021/bi00789a030 · Cited by 8,526
Vascular nitric oxide: Beyond eNOS
Zhao, Y., Vanhoutte, P. M., & Leung, S. W. S. (2015) · Journal of Pharmacological Sciences · doi:10.1016/j.jphs.2015.09.002 · Cited by 875
Metabolic dependence of red cell deformability
Weed, R. I., LaCelle, P. L., & Merrill, E. W. (1969) · Journal of Clinical Investigation · doi:10.1172/jci106038 · Cited by 818
Self-citationStructure of Biological Membranes
Korn, E. D. (1966) · Science · doi:10.1126/science.153.3743.1491 · Cited by 547
Characterization and quantification of red cell lipids in normal man
Ways, P. O., & Hanahan, D. J. (1964) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40200-7 · Cited by 472
Metadata
- Authors
- Weed, R. I., Reed, C. F., & Berg, G.
- Affiliations
- University of Rochester Medicine (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1963-04-01
- Volume
- 42
- Issue
- 4
- Pages
- 581-588
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC289318
- OpenAlex
- W2161381317
- Cited by
- 240
- References
- 32
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 7 · 2023: 9 · 2022: 6 · 2021: 10 · 2020: 15 · 2019: 4 · 2018: 10 · 2017: 8 · 2016: 7 · 2015: 12 · 2014: 4 · 2013: 11 · 2012: 2
- FWCI
- 9.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University of Rochester
- Topics
- Health Sciences › Medicine › Physiology (medical) › Erythrocyte Function and Pathophysiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Health Sciences › Medicine › Genetics (clinical) › Hemoglobinopathies and Related Disorders
- Keywords
- hemoglobin, erythrocyte membrane, membrane integrity, erythrocyte ghosts, osmotic lysis, membrane lipid composition, erythrocyte shape
- MeSH terms
- Erythrocyte Membrane, Erythrocytes, Hemoglobins, Humans
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