The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Stimulation of collagen gene expression and protein synthesis in murine mesangial cells by high glucose is mediated by autocrine activation of transforming growth factor-beta.
Open accessAbstract
Previous investigations have demonstrated that growing mesangial cells in high glucose concentration stimulates extracellular matrix synthesis and also increases the expression of TGF-beta. We tested whether the stimulation of extracellular matrix production is mediated by autocrine activation of TGF-beta, a known prosclerotic cytokine. Addition of neutralizing anti-TGF-beta antibody, but not normal rabbit IgG, significantly reduced the high glucose-stimulated incorporation of 3[H]proline. Denaturing SDS-PAGE revealed that mainly collagen types I and IV were stimulated by high (450 mg/dl) D-glucose. This high glucose-mediated increase in collagen synthesis was reduced by the anti-TGF-beta antibody. Treatment of mesangial cells grown in normal (100 mg/dl) D-glucose with 2 ng/ml recombinant TGF-beta 1 mimicked the effects of high glucose. Furthermore, the anti-TGF-beta antibody significantly reduced the increase in mRNA levels encoding alpha 2(I) and alpha 1(IV) collagens induced by high glucose. Thus, the high glucose-stimulated increase of collagen production in mesangial cells is mediated, at least in part, by autocrine TGF-beta activation. We postulate that the interception of the glomerular activity of TGF-beta may be an effective intervention in the management of diabetic nephropathy.
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Okuda, S., Languino, L. R., Ruoslahti, E., & Border, W. A. (1990) · Journal of Clinical Investigation · doi:10.1172/jci114731 · Cited by 546
Overexpression of Fibronectin Induced by Diabetes or High Glucose: Phenomenon With a Memory
Roy, S., Sala, R., Cagliero, E., & Lorenzi, M. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.1.404 · Cited by 409
Cited by
Biochemistry and molecular cell biology of diabetic complications
Brownlee, M. (2001) · Nature · doi:10.1038/414813a · Cited by 9,107
The role of protein kinase C activation and the vascular complications of diabetes
DASEVCIMEN, N., & KING, G. (2007) · Pharmacological Research · doi:10.1016/j.phrs.2007.04.016 · Cited by 494
The Pathogenesis of Myocardial Fibrosis in the Setting of Diabetic Cardiomyopathy
Asbun, J., & Villarreal, F. J. (2006) · Journal of the American College of Cardiology · doi:10.1016/j.jacc.2005.09.050 · Cited by 486
Russo, I., & Frangogiannis, N. G. (2016) · Journal of Molecular and Cellular Cardiology · doi:10.1016/j.yjmcc.2015.12.011 · Cited by 474
Mediators of Diabetic Renal Disease: The Case for TGF-β as the Major Mediator
Ziyadeh, F. N. (2004) · Journal of the American Society of Nephrology · doi:10.1097/01.asn.0000093460.24823.5b · Cited by 463
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Metadata
- Authors
- Ziyadeh, F. N., Sharma, K., Ericksen, M., & Wolf, G.
- Venue
- Journal of Clinical Investigation
- Published
- 1994-02-01
- Volume
- 93
- Issue
- 2
- Pages
- 536-542
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2145356697
- Cited by
- 597
- References
- 2
- FWCI
- 18.34
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Ziyadeh, F. N., Sharma, K., Ericksen, M., & Wolf, G. (1994). Stimulation of collagen gene expression and protein synthesis in murine mesangial cells by high glucose is mediated by autocrine activation of transforming growth factor-beta. Journal of Clinical Investigation. https://doi.org/10.1172/jci117004
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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