Identification of cardiac myosin peptides capable of inducing autoimmune myocarditis in BALB/c mice.
Open accessAbstract
Immunization with cardiac myosin induces T cell-mediated myocarditis in genetically predisposed mice and serves as a model for autoimmune heart disease. This study was undertaken to identify pathogenic epitopes on the myosin molecule. Our approach was based on the comparison of the pathogenicity between cardiac (alpha-)myosin and soleus muscle (beta-)myosin. We show that alpha-myosin is the immunodominant isoform and induces myocarditis at high severity and prevalence whereas beta-myosin induces little disease. Therefore the immunodominant epitopes of alpha-myosin must reside in regions of different amino acid sequence between alpha- and beta-myosin isoforms. Cardiac myosin peptides corresponding to these regions of difference were synthesized and tested for their ability to induce inflammatory heart disease. Three pathogenic peptides were identified. One peptide that is located in the head portion of the molecule induced severe myocarditis, whereas two others that reside in the rod portion possessed only minor pathogenicity. The identification of pathogenic epitopes on the cardiac myosin molecule will allow detailed studies on the recognition of this antigen by the immune system and might be used to downmodulate ongoing heart disease.
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Immunization with cardiac myosin or its shortened peptide in mice or rats can induce experimental autoimmune myocarditis.
“immunization with cardiac myosin or its shortened peptide in mice or rats could induce the development of experimental autoimmune myocarditis (EAM)”
From Autoimmune Myocarditis, Old Dogs and New Tricks. (2024) · cites this paper as [66]
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Research works printed but not linked: 2
- Woodruff, J.F. 1980. Viral myocarditis: a review. Am. J. Pathol. 101:427- 484.
- Smith, S.C., and P.M. Allen. 1992. Expression of myosin-class II major histocompatibility complexes in the normal myocardium occurs before induc- tion of autoimmune myocarditis. Proc. Natl. Acad. Sci. USA. 89:9131-9135.
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- Authors
- Pummerer, C. L., Luze, K., Grässl, G., Bachmaier, K., Offner, F., Burrell, S. K., Lenz, D. M., Zamborelli, T. J., Penninger, J. M., & Neu, N.
- Affiliations
- Innsbruck Medical University (AT)
- Amgen (United States) (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1996-05-01
- Volume
- 97
- Issue
- 9
- Pages
- 2057-2062
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC507280
- OpenAlex
- W2059277480
- Cited by
- 205
- References
- 0
- Citations by year
- 2026: 7 · 2025: 9 · 2024: 9 · 2023: 6 · 2022: 4 · 2021: 8 · 2020: 5 · 2019: 7 · 2018: 5 · 2017: 10 · 2016: 7 · 2015: 10 · 2014: 6 · 2013: 10 · 2012: 3
- FWCI
- 4.06
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
- Health Sciences › Medicine › Ophthalmology › Ocular Diseases and Behçet’s Syndrome
- Keywords
- cardiac myosin, autoimmune myocarditis, epitope identification, immunodominant epitopes, soleus muscle, myosin head, myosin rod, BALB/c mice
- MeSH terms
- Amino Acid Sequence, Animals, Autoimmune Diseases, Immunization, Mice, Inbred BALB C, Molecular Sequence Data, Myocarditis, Myocardium, Myosins, Peptide Fragments, Epitope Mapping, Mice, Rats
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