Hostile takeovers: viral appropriation of the NF-kB pathway

Abstract

NF-κB in defense and diseaseTranscriptional regulators of the NF-κB/IκB family promote the expression of well over 100 target genes, the majority of which participate in the host immune response (1).These proteins include a multitude of cytokines and chemokines, receptors required for immune recognition, proteins involved in antigen presentation, and adhesion receptors involved in transmigration across blood vessels walls.Because of this extensive role in immune action, NF-κB has been termed the central mediator of the immune response.Gene knockout and other studies establish roles for NF-κB in the ontogeny of the immune system but also demonstrate that NF-κB participates at multiple steps during oncogenesis (2) and the regulation of programmed cell death (3).For several reasons, the NF-κB pathway provides an attractive target to viral pathogens.Activation of NF-κB is a rapid, immediate early (IE) event that occurs within minutes after exposure to a relevant inducer, does not require de novo protein synthesis, and results in a strong transcriptional stimulation of several early viral as well as cellular genes.In this review, we will describe strategies that viruses have evolved to modulate the NF-κB pathway, to enhance viral replication, host cell survival, and evasion of immune responses.Activation of NF-κB constitutes an obvious target because many of its target genes -growth factors, cytokines and their receptors, and protooncogenes -profoundly influence the host cell cycle.In addition, some viruses exploit the antiapoptotic properties of NF-κB to evade the host defense mechanisms that limit replication by killing infected cells, or conversely to trigger apoptosis as a mechanism to increase virus spread.Perhaps not surprisingly, the persistent activation of the NF-κB pathway maintained by certain viruses contributes to oncogenic transformation (2).In addition to the classic studies with the avian REV-T retrovirus which contains the v-Rel oncoprotein and induces a rapid and fatal B-cell lymphoma in young birds (4), several lines of evidence demonstrate that NF-κB family members contribute to human oncogenesis.Localization of NF-κB-encoding genes at sites of chromosomal translocations and genomic rearrangements in cancer, high levels of NF-κB activity in many breast cancer cells, and constitutive nuclear NF-κB complexes in Hodgkin's lymphoma cells all support this view (2).Furthermore, as discussed below, viral oncogene products, including human T-cell leukemia virus type 1 (HTLV-1) Tax protein and Epstein-Barr virus latent infection membrane protein 1 (EBV LMP1), each act by unique mechanisms to disrupt NF-κB regulation and initiate viral transformation.