The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Identification of functional endothelial protein C receptor in human plasma.
Open accessAbstract
The endothelial cell protein C receptor (EPCR) binds protein C and facilitates activation by the thrombin-thrombomodulin complex. EPCR also binds activated protein C (APC) and inhibits APC anticoagulant activity. In this study, we detected a soluble form of EPCR in normal human plasma. Plasma EPCR appears to be approximately 43, 000 D, and circulates at approximately 100 ng/ml (98.4+/-27.8 ng/ml, n = 22). Plasma EPCR was purified from human citrated plasma using ion exchange, immunoaffinity, and protein C affinity chromatography. Flow cytometry experiments demonstrated that plasma EPCR bound APC with an affinity similar to that previously determined for recombinant soluble EPCR (Kdapp = 30 nM). Furthermore, plasma EPCR inhibited both protein C activation on an endothelial cell line and APC anticoagulant activity in a one-stage Factor Xa clotting assay. The physiological function of plasma EPCR is uncertain, but if the local concentrations are sufficiently high, particularly in disease states, the present data suggest that the soluble plasma EPCR could attenuate the membrane-bound EPCR augmentation of protein C activation and the anticoagulant function of APC.
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“Research has shown that soluble EPCR impairs the activation of protein C. 58”
From Meta-analysis of ACE inhibitor-induced angioedema identifies novel risk locus. (2024) · cites this paper as [58]
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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311
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Edgell, C. J., McDonald, C., & Graham, J. B. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.12.3734 · Cited by 1,495
Identification of an endothelial cell cofactor for thrombin-catalyzed activation of protein C.
Esmon, C. T., & Owen, W. G. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.4.2249 · Cited by 831
Self-citationProtein C prevents the coagulopathic and lethal effects of Escherichia coli infusion in the baboon.
Taylor, F. B., Chang, A. C. K., Esmon, C. T., D’Angelo, A., Vigano’D’Angelo, S., & Blick, K. E. (1987) · Journal of Clinical Investigation · doi:10.1172/jci112902 · Cited by 820
Self-citationStearns-Kurosawa, D. J., Kurosawa, S., Mollica, J. S., Ferrell, G. L., & Esmon, C. T. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.19.10212 · Cited by 528
Self-citationFukudome, K., & Esmon, C. T. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47220-1 · Cited by 516
Self-citationEsmon, C. T., Esmon, N. L., & Harris, K. W. (1982) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)34276-5 · Cited by 432
Self-citationGlycosaminoglycans and the regulation of blood coagulation
Bourin, M., & Lindahl, U. (1993) · Biochemical Journal · doi:10.1042/bj2890313 · Cited by 420
Plasma thrombomodulin in health and diseases
Takano, S., Kimura, S., Ohdama, S., & Aoki, N. (1990) · Blood · doi:10.1182/blood.v76.10.2024.bloodjournal76102024 · Cited by 377
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Dysfunction of Endothelial Protein C Activation in Severe Meningococcal Sepsis
Faust, S. N., Levin, M., Harrison, O. B., Goldin, R. D., Lockhart, M. S., Kondaveeti, S., Laszik, Z. G., Esmon, C. T., & Heyderman, R. S. (2001) · New England Journal of Medicine · doi:10.1056/nejm200108093450603 · Cited by 694
Self-citationCandidate‐based proteomics in the search for biomarkers of cardiovascular disease
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Thrombomodulin-Protein C-EPCR System
de Wouwer, M. V., Collen, D., & Conway, E. M. (2004) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000134298.25489.92 · Cited by 375
Thrombomodulin and its role in inflammation
Conway, E. M. (2011) · Seminars in Immunopathology · doi:10.1007/s00281-011-0282-8 · Cited by 329
The endothelial cell protein C receptor aids in host defense against Escherichia coli sepsis
Taylor, F. B., Stearns-Kurosawa, D. J., Kurosawa, S., Ferrell, G. L., Chang, A. C. K., Laszik, Z. G., Kosanke, S. D., Peer, G., & Esmon, C. T. (2000) · Blood · doi:10.1182/blood.v95.5.1680.005k33_1680_1686 · Cited by 298
Self-citation
Metadata
- Authors
- Kurosawa, S., Stearns-Kurosawa, D. J., Hidari, N., & Esmon, C. T.
- Affiliations
- Oklahoma Medical Research Foundation (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Esmon, C T
- Venue
- Journal of Clinical Investigation
- Published
- 1997-07-15
- Volume
- 100
- Issue
- 2
- Pages
- 411-418
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC508205
- OpenAlex
- W1993601122
- Cited by
- 154
- References
- 50
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2022: 3 · 2021: 2 · 2020: 2 · 2017: 3 · 2016: 8 · 2015: 8 · 2014: 13 · 2013: 16 · 2012: 2
- FWCI
- 3.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health (P50 HL54502)
- National Heart, Lung, and Blood Institute (P50HL054502, P01 HL54804, P50-HL54502, P01HL054804)
- Topics
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Keywords
- endothelial protein C receptor, soluble EPCR, activated protein C, protein C activation, anticoagulant activity, thrombin-thrombomodulin complex, plasma protein purification, endothelial cells
- MeSH terms
- Anticoagulants, Blood Coagulation, Blood Coagulation Factors, Cells, Cultured, Chromatography, Affinity, Endothelium, Vascular, Flow Cytometry, Humans, Protein Binding, Protein C, Receptors, Cell Surface, Recombinant Proteins, Solubility, Thrombin, Blotting, Western, Factor Xa, Thrombomodulin
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- Kurosawa, S., Stearns-Kurosawa, D. J., Hidari, N., & Esmon, C. T. (1997). Identification of functional endothelial protein C receptor in human plasma. Journal of Clinical Investigation. https://doi.org/10.1172/jci119548
