The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Mast cell tryptase regulates rat colonic myocytes through proteinase-activated receptor 2.
Open accessAbstract
Proteinase-activated receptor-2 (PAR-2) is a G protein-coupled receptor that is cleaved and activated by trypsin-like enzymes. PAR-2 is highly expressed by small intestinal enterocytes where it is activated by luminal trypsin. The location, mechanism of activation, and biological functions of PAR-2 in the colon, however, are unknown. We localized PAR-2 to the muscularis externa of the rat colon by immunofluorescence. Myocytes in primary culture also expressed PAR-2, assessed by immunofluorescence and RT-PCR. Trypsin, SLIGRL-NH2 (corresponding to the PAR-2 tethered ligand), mast cell tryptase, and a filtrate of degranulated mast cells stimulated a prompt increase in [Ca2+]i in myocytes. The response to tryptase and the mast cell filtrate was inhibited by the tryptase inhibitor BABIM, and abolished by desensitization of PAR-2 with trypsin. PAR-2 activation inhibited the amplitude of rhythmic contractions of strips of rat colon. This response was unaffected by indomethacin, l-NG-nitroarginine methyl ester, a bradykinin B2 receptor antagonist and tetrodotoxin. Thus, PAR-2 is highly expressed by colonic myocytes where it may be cleaved and activated by mast cell tryptase. This may contribute to motility disturbances of the colon during conditions associated with mast cell degranulation.
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Cites
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Interactions of Mast Cell Tryptase with Thrombin Receptors and PAR-2
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Molecular cloning, expression and potential functions of the human proteinase-activated receptor-2
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Cited by
Electrolyte transport in the mammalian colon: mechanisms and implications for disease
Kunzelmann, K., & Mall, M. (2002) · Physiological Reviews · doi:10.1152/physrev.00026.2001 · Cited by 655
Mast cell tryptases and chymases in inflammation and host defense.
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Self-citationProtease-activated receptors in inflammation, neuronal signaling and pain
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Self-citationMast Cell Tryptase Controls Paracellular Permeability of the Intestine
Jacob, C., Yang, P.-C., Darmoul, D., Amadesi, S., Saito, T., Cottrell, G. S., Coelho, A.-M., Singh, P., Grady, E. F., Perdue, M., et al. (2005) · Journal of Biological Chemistry · doi:10.1074/jbc.m506338200 · Cited by 334
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Metadata
- Authors
- Corvera, C. U., Déry, O., McConalogue, K., Böhm, S. K., Khitin, L. M., Caughey, G. H., Payan, D. G., & Bunnett, N. W.
- Venue
- Journal of Clinical Investigation
- Published
- 1997-09-15
- Volume
- 100
- Issue
- 6
- Pages
- 1383-1393
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W1963543751
- Cited by
- 289
- References
- 35
- FWCI
- 6.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Corvera, C. U., Déry, O., McConalogue, K., Böhm, S. K., Khitin, L. M., Caughey, G. H., Payan, D. G., & Bunnett, N. W. (1997). Mast cell tryptase regulates rat colonic myocytes through proteinase-activated receptor 2. Journal of Clinical Investigation. https://doi.org/10.1172/jci119658
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