The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Biosynthesis of 15-deoxy-Δ12,14-PGJ2 and the ligation of PPARγ
Open accessAbstract
15-deoxy-Delta12,14-PGJ2 (15d-PGJ2) has been identified as an endogenous ligand for PPARgamma, inducing adipogenesis in vitro. Additional roles for this molecule in the propagation and resolution of inflammation, ligation of NF-kappaB, and mediation of apoptosis have been proposed. However, quantitative, physiochemical evidence for the formation of 15d-PGJ2 in vivo is lacking. We report that 15d-PGJ2 is detectable using liquid chromatography-mass spectrometry-mass spectrometry at low picomolar concentrations in the medium of 3T3-L1 preadipocytes. However, despite induction of COX-2, production of PGs, including 15d-PGJ2, does not increase during adipocyte differentiation, a process unaltered by COX inhibition. 15d-PGJ2 is detectable as a minor product of COX-2 in human urine. However, its biosynthesis is unaltered during or after COX activation in vivo by LPS. Furthermore, the biosynthesis of 15d-PGJ2 is not augmented in the joint fluid of patients with arthritis, nor is its urinary excretion increased in patients with diabetes or obesity. 15d-PGJ2 is not the endogenous mediator of PPARgamma-dependent adipocyte activation and is unaltered in clinical settings in which PPARgamma activation has been implicated.
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Macrophages, inflammation, and insulin resistance
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Metadata
- Authors
- Bell-Parikh, L. C., Ide, T., Lawson, J. A., McNamara, P., Reilly, M., & FitzGerald, G. A.
- Venue
- Journal of Clinical Investigation
- Published
- 2003-09-15
- Volume
- 112
- Issue
- 6
- Pages
- 945-955
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2093584890
- Cited by
- 341
- References
- 5
- FWCI
- 10.94
- Type
- journal article
- Language
- en
- Open access
- bronze
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
