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Nephritogenic mAb 5-1-6 is directed at the extracellular domain of rat nephrin
Open accessAbstract
mAb 5-1-6 identifies an antigen on rat podocyte slit-diaphragms and induces severe proteinuria when injected into rats. Nephrin, an Ig-like transmembrane protein that is mutated in congenital nephrotic syndrome of the Finnish type, has been localized to the slit-diaphragm on human podocytes. Here we document that the mAb 5-1-6 antigen is rat nephrin. After incubation of rat glomeruli with this mAb, the antibody/antigen complex was chemically cross-linked, extracted, and immunoprecipitated, prior to Western analysis. By mass spectrometry and 2D gel electrophoresis, we identified several peptides with complete identity to human nephrin. In addition, the 185-kDa protein immunoprecipitated by mAb 5-1-6 from rat glomerular extracts reacts with a rabbit anti-mouse nephrin antibody. Finally, nephrin and the mAb 5-1-6 antigen have identical glomerular localization patterns on immunofluorescence of rat kidney. These results demonstrate that the nephritogenic mAb 5-1-6 identifies the extracellular domain of nephrin, thereby documenting the importance of the slit-diaphragm and its component, nephrin, in the regulation of glomerular permselectivity.
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Citations
Cited by
Cell biology of the glomerular podocyte
Pavenstädt, H., Kriz, W., & Kretzler, M. (2003) · Physiological Reviews · doi:10.1152/physrev.00020.2002 · Cited by 1,499
The podocyte’s response to injury: role in proteinuria and glomerulosclerosis
Shankland, S. J. (2006) · Kidney International · doi:10.1038/sj.ki.5000410 · Cited by 846
Patrakka, J., Kestilä, M., Wartiovaara, J., Ruotsalainen, V., Tissari, P., Lenkkeri, U., Männikkö, M., Visapää, I., Holmberg, C., Rapola, J., et al. (2000) · Kidney International · doi:10.1046/j.1523-1755.2000.00254.x · Cited by 310
Doublier, S., Ruotsalainen, V., Salvidio, G., Lupia, E., Biancone, L., Conaldi, P. G., Reponen, P., Tryggvason, K., & Camussi, G. (2001) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)64128-4 · Cited by 257
Metadata
- Authors
- Topham, P. S., Kawachi, H., Haydar, S. A., Chugh, S., Addona, T. A., Charron, K. B., Holzman, L. B., Shia, M., Shimizu, F., & Salant, D. J.
- Venue
- Journal of Clinical Investigation
- Published
- 1999-12-01
- Volume
- 104
- Issue
- 11
- Pages
- 1559-1566
- ISSN
- 0021-9738 (ISSN-L)
- OpenAlex
- W2165581077
- Cited by
- 199
- References
- 0
- FWCI
- 10.08
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Nephritogenic mAb 5-1-6 is directed at the extracellular domain of rat nephrin. https://app.cochat.ai/papers/10.1172/jci7728
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- Topham, P. S., Kawachi, H., Haydar, S. A., Chugh, S., Addona, T. A., Charron, K. B., Holzman, L. B., Shia, M., Shimizu, F., & Salant, D. J. (1999). Nephritogenic mAb 5-1-6 is directed at the extracellular domain of rat nephrin. Journal of Clinical Investigation. https://doi.org/10.1172/jci7728
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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