The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue
Open accessAbstract
The fluorescent pigment lipofuscin accumulates with age in the cytoplasm of cells of the CNS. Because of its broad excitation and emission spectra, the presence of lipofuscin-like autofluorescence complicates the use of fluorescence microscopy (e.g., fluorescent retrograde tract tracing and fluorescence immunocytochemistry). In this study we examined several chemical treatments of tissue sections for their ability to reduce or eliminate lipofuscin-like autofluorescence without adversely affecting other fluorescent labels. We found that 1-10 mM CuSO 4 in 50 mM ammonium acetate buffer (pH 5) or 1% Sudan Black B (SB) in 70% ethanol reduced or eliminated lipofuscin autofluorescence in sections of monkey, human, or rat neural tissue. These treatments also slightly reduced the intensity of immunofluorescent labeling and fluorescent retrograde tract tracers. However, the reduction of these fluorophores was far less dramatic than that for the lipofuscin-like compound. We conclude that treatment of tissue with CuSO 4 or SB provides a reasonable compromise between reduction of lipofuscin-like fluorescence and maintenance of specific fluorescent labels.
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Cites
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Age-related myelin degradation burdens the clearance function of microglia during aging
Safaiyan, S., Kannaiyan, N., Snaidero, N., Brioschi, S., Biber, K., Yona, S., Edinger, A. L., Jung, S., Rossner, M. J., & Simons, M. (2016) · Nature Neuroscience · doi:10.1038/nn.4325 · Cited by 604
Transplanted bone marrow generates new neurons in human brains
Mezey, É., Key, S., Vogelsang, G., Szalayova, I., Lange, G. D., & Crain, B. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0336479100 · Cited by 567
Molecular interrogation of hypothalamic organization reveals distinct dopamine neuronal subtypes
Romanov, R. A., Zeisel, A., Bakker, J., Girach, F., Hellysaz, A., Tomer, R., Alpár, A., Mulder, J., Clotman, F., Keimpema, E., et al. (2017) · Nature Neuroscience · doi:10.1038/nn.4462 · Cited by 502
Metadata
- Authors
- Schnell, S. A., Staines, W. A., & Wessendorf, M. W.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1999-06
- Volume
- 47
- Issue
- 6
- Pages
- 719-730
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2003179114
- Cited by
- 688
- References
- 29
- FWCI
- 8.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Schnell, S. A., Staines, W. A., & Wessendorf, M. W. (1999). Reduction of Lipofuscin-like Autofluorescence in Fluorescently Labeled Tissue. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/002215549904700601
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
