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A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE
Abstract
A method is described for the fixation of blocks of tissue for use in studies employing immunofluorescence. This method consists of fixing thin blocks in 95% ethanol and carrying out the subsequent dehydration and clearing at refrigerator temperatures (4°C). Thereafter, embedding in paraffin and sectioning by the standard microtomy is easy. This method results in preparations which are histologically more precise in the localization of antigen or antibody than preparations of frozen tissues; and with rabbit antibody and bovine serum albumin, the sensitivity of detection is enhanced. Bovine serum albumin can be found for longer periods after injection than is possible with frozen sections. Other antigens for which this procedure has proved satisfactory are bovine gamma globulin, horse ferritin, influenza A virus, diphtheria and tetanus toxoids. Hen's ovalbumin deteriorated. New antigens or new antibodies should be tested before being committed to this method.
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Cited by
Late cutaneous allergic responses in isolated IgE-dependent reactions
Dolovich, J., Hargreave, F. E., Chalmers, R., Shier, K. J., Gauldie, J., & Bienenstock, J. (1973) · Journal of Allergy and Clinical Immunology · doi:10.1016/0091-6749(73)90119-x · Cited by 451
Appearance and distribution of collagens and laminin in the early mouse embryo
Leivo, I., Vaheri, A., Timpl, R., & Wartiovaara, J. (1980) · Developmental Biology · doi:10.1016/0012-1606(80)90365-6 · Cited by 398
The gut‐associated lymphoid system: nature and properties of the large dividing cells
Guy‐Grand, D., Griscelli, C., & Vassali, P. (1974) · European Journal of Immunology · doi:10.1002/eji.1830040610 · Cited by 386
Immunomodulating capacity of kefir
Vinderola, C. G., Duarte, J., Thangavel, D., Perdigón, G., Farnworth, E., & Matar, C. (2005) · Journal of Dairy Research · doi:10.1017/s0022029905000828 · Cited by 210
LeBlanc, J. G., Matar, C., Valdéz, J. C., LeBlanc, J., & Perdigon, G. (2002) · Journal of Dairy Science · doi:10.3168/jds.s0022-0302(02)74360-9 · Cited by 210
Metadata
- Authors
- SAINTE-MARIE, G.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1962-05
- Volume
- 10
- Issue
- 3
- Pages
- 250-256
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2043795458
- Cited by
- 1,108
- References
- 0
- FWCI
- 22.89
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE. https://app.cochat.ai/papers/10.1177/10.3.250
- The paper
- SAINTE-MARIE, G. (1962). A PARAFFIN EMBEDDING TECHNIQUE FOR STUDIES EMPLOYING IMMUNOFLUORESCENCE. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/10.3.250
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
