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Fluorescence Polarization and Anisotropy in High Throughput Screening: Perspectives and Primer
Open accessAbstract
Fluorescence polarization and anisotropy are two nearly equivalent techniques that have together, over the past 5 years, achieved wide use in high throughput screening in drug discovery. These are single-label methods that can be used to construct homogeneous assays that are fast, sensitive, and resistant to some significant interferences. Moreover, the assays are relatively inexpensive. This review surveys the peer-reviewed literature on the subject and explores some of the fundamental issues that bear on assay performance.
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Cited by
Fluorescent Probes to Characterise FK506‐Binding Proteins
Kozany, C., März, A., Kress, C., & Hausch, F. (2009) · ChemBioChem · doi:10.1002/cbic.200800806 · Cited by 91
Advancing Targeted Protein Degradation via Multiomics Profiling and Artificial Intelligence
Duran-Frigola, M., Cigler, M., & Winter, G. E. (2023) · Journal of the American Chemical Society · doi:10.1021/jacs.2c11098 · Cited by 50
Golden, M. S., Cote, S. M., Sayeg, M., Zerbe, B. S., Villar, E. A., Beglov, D., Sazinsky, S. L., Georgiadis, R. M., Vajda, S., Kozakov, D., et al. (2013) · Journal of the American Chemical Society · doi:10.1021/ja400914z · Cited by 47
Screening-based approaches to identify small molecules that inhibit protein–protein interactions
Choi, S., & Choi, K.-Y. (2017) · Expert Opinion on Drug Discovery · doi:10.1080/17460441.2017.1280456 · Cited by 29
Metadata
- Authors
- Owicki, J. C.
- Venue
- SLAS Discovery
- Publisher
- Elsevier BV
- Published
- 2000-10
- Volume
- 5
- Issue
- 5
- Pages
- 297-306
- ISSN
- 2472-5552 (ISSN-L)
- OpenAlex
- W1964381872
- Cited by
- 303
- References
- 0
- FWCI
- 5.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Owicki, J. C. (2000). Fluorescence Polarization and Anisotropy in High Throughput Screening: Perspectives and Primer. SLAS Discovery. https://doi.org/10.1177/108705710000500501
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