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The Expression of Dentin Sialophosphoprotein Gene in Bone

Qin, C., Brunn, J.C., Cadena, E., Ridall, A., Tsujigiwa, H., Nagatsuka, H., Nagai, N., Butler, W.T.·2002·Journal of Dental Research

Abstract

Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript coding for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be tooth-specific. To test for the expression of the dspp gene in bone, we performed Western immunoblots and reverse-transcription polymerase chain-reaction (RT-PCR). With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using RT-PCR, we detected DSPP mRNA in mouse calvaria. Similar to Western immunoblots, the results of RT-PCR indicated that the dspp gene is expressed at a lower level in bone than in dentin and odontoblasts. Analysis of the data shows that DSPP is not a tooth-specific protein, and that dramatically different regulatory mechanisms governing DSPP expression are involved in the bone and dentin.

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Metadata

Authors
Qin, C., Brunn, J. C., Cadena, E., Ridall, A., Tsujigiwa, H., Nagatsuka, H., Nagai, N., & Butler, W. T.
Venue
Journal of Dental Research
Published
2002-06
Volume
81
Issue
6
Pages
392-394
ISSN
  • 0022-0345 (ISSN-L)
  • 1544-0591
OpenAlex
W2123779499
Cited by
264
References
16
FWCI
7.80
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: The Expression of Dentin Sialophosphoprotein Gene in Bone. https://app.cochat.ai/papers/10.1177/154405910208100607
The paper
Qin, C., Brunn, J. C., Cadena, E., Ridall, A., Tsujigiwa, H., Nagatsuka, H., Nagai, N., & Butler, W. T. (2002). The Expression of Dentin Sialophosphoprotein Gene in Bone. Journal of Dental Research. https://doi.org/10.1177/154405910208100607

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