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Cited by 117Tissue Engineering and Regenerative Medicine3D Printing in Biomedical ResearchCardiac Valve Diseases and Treatments

Recellularization of decellularized heart valves: Progress toward the tissue-engineered heart valve

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VeDepo, Mitchell C, Detamore, Michael S, Hopkins, Richard A, Converse, Gabriel L·2017·Journal of Tissue Engineering
DOI 10.1177/2041731417726327PMC PMC5574480
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Abstract

The tissue-engineered heart valve portends a new era in the field of valve replacement. Decellularized heart valves are of great interest as a scaffold for the tissue-engineered heart valve due to their naturally bioactive composition, clinical relevance as a stand-alone implant, and partial recellularization in vivo. However, a significant challenge remains in realizing the tissue-engineered heart valve: assuring consistent recellularization of the entire valve leaflets by phenotypically appropriate cells. Many creative strategies have pursued complete biological valve recellularization; however, identifying the optimal recellularization method, including in situ or in vitro recellularization and chemical and/or mechanical conditioning, has proven difficult. Furthermore, while many studies have focused on individual parameters for increasing valve interstitial recellularization, a general understanding of the interacting dynamics is likely necessary to achieve success. Therefore, the purpose of this review is to explore and compare the various processing strategies used for the decellularization and subsequent recellularization of tissue-engineered heart valves.

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  • Hypoxic bioreactor conditioning increases cellular infiltration in decellularized heart valve leaflets.

    “Initiatives to develop decellularized heart valves with anti‐calcification properties have been undertaken, with hypoxic bioreactor conditioning demonstrating increased cellular infiltration in valve leaflets.[ 169 , 170 ]”

    From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [170]

    The In vitro recellularization section (page unavailable) reports greater leaflet cell infiltration after cyclic pressure conditioning in a bioreactor than after static culture. It describes mechanical pressure conditioning, not hypoxic conditioning; the claimed role of hypoxia is unsupported. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

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References 144 · Cited by 117

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Authors
VeDepo, M. C., Detamore, M. S., Hopkins, R. A., & Converse, G. L.
Affiliations
  • Children's Mercy Hospital (US)
  • University of Oklahoma (US)
Corresponding author
Converse, Gabriel L
ORCID
Venue
Journal of Tissue Engineering
Publisher
SAGE Publications
Published
2017-01-01
Volume
8
Pages
2041731417726327-2041731417726327
ISSN
2041-7314 (ISSN-L)
DOI
10.1177/2041731417726327
PMC
PMC5574480
OpenAlex
W2748725547
Cited by
117
References
144
Citations by year
2026: 4 · 2025: 8 · 2024: 17 · 2023: 11 · 2022: 10 · 2021: 19 · 2020: 24 · 2019: 12 · 2018: 11 · 2017: 1
FWCI
2.78
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Tissue Engineering (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Valve Diseases and Treatments
Keywords
tissue-engineered heart valves, decellularized heart valves, recellularization, valve leaflets, valvular interstitial cells, mechanical conditioning, chemical conditioning, heart valve replacement

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