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Cited by 42Electrospun Nanofibers in Biomedical ApplicationsWound Healing and Treatmentsbiodegradable polymer synthesis and properties

Prevention of excessive scar formation using nanofibrous meshes made of biodegradable elastomer poly(3-hydroxybutyrate- co -3-hydroxyvalerate)

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Kim, Hye Sung, Chen, Junyu, Wu, Lin-Ping, Wu, Jihua, Xiang, Hua, Leong, Kam W, Han, Jing·2020·Journal of Tissue Engineering
DOI 10.1177/2041731420949332PMC PMC7448259
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Abstract

To reduce excessive scarring in wound healing, electrospun nanofibrous meshes, composed of haloarchaea-produced biodegradable elastomer poly(3-hydroxybutyrate- co-3-hydroxyvalerate) (PHBV), are fabricated for use as a wound dressing. Three PHBV polymers with different 3HV content are used to prepare either solution-cast films or electrospun nanofibrous meshes. As 3HV content increases, the crystallinity decreases and the scaffolds become more elastic. The nanofibrous meshes exhibit greater elasticity and elongation at break than films. When used to culture human dermal fibroblasts in vitro, PHBV meshes give better cell attachment and proliferation, less differentiation to myofibroblasts, and less substrate contraction. In a full-thickness mouse wound model, treatment with films or meshes enables regeneration of pale thin tissues without scabs, dehydration, or tubercular scar formation. The epidermis of wounds treated with meshes develop small invaginations in the dermis within 2 weeks, indicating hair follicle and sweat gland regeneration. Consistent with the in vitro results, meshes reduce myofibroblast differentiation in vivo through downregulation of α-SMA and TGF-β1, and upregulation of TGF-β3. The regenerated wounds treated with meshes are softer and more elastic than those treated with films. These results demonstrate that electrospun nanofibrous PHBV meshes mitigate excessive scar formation by regulating myofibroblast formation, showing their promise for use as wound dressings.

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Cited by

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Authors
Kim, H. S., Chen, J., Wu, L.-P., Wu, J., Xiang, H., Leong, K. W., & Han, J.
Affiliations
  • Columbia University (US)
  • Chinese Academy of Sciences (CN)
  • Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
  • PLA Strategic Support Force Characteristic Medical Center, Beijing, China
Corresponding author
Han, Jing
ORCID
Venue
Journal of Tissue Engineering
Publisher
SAGE Publications
Published
2020-01
Volume
11
Pages
2041731420949332-2041731420949332
ISSN
2041-7314 (ISSN-L)
DOI
10.1177/2041731420949332
PMC
PMC7448259
OpenAlex
W3081058573
Cited by
42
References
0
Citations by year
2026: 7 · 2025: 8 · 2024: 6 · 2023: 8 · 2022: 8 · 2021: 4 · 2020: 1
FWCI
1.34
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Tissue Engineering (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • national basic research program of china (2018YFA0900200, 2019YFA0110500)
  • national research foundation of korea (2016R1A6A3A03012178)
  • National Natural Science Foundation of China (31970031 and 91751201)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Keywords
scar prevention, poly(3-hydroxybutyrate-co-3-hydroxyvalerate), biodegradable elastomers, wound dressings, myofibroblast differentiation, TGF-β1, TGF-beta3, alpha-smooth muscle actin, full-thickness wound healing, tissue regeneration, hair follicle regeneration

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