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Cited by 51Tissue Engineering and Regenerative MedicineBone Tissue Engineering MaterialsCollagen: Extraction and Characterization

Three dimensional porous scaffolds derived from collagen, elastin and fibrin proteins orchestrate adipose tissue regeneration

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Sawadkar, Prasad, Mandakhbayar, Nandin, Patel, Kapil D, Buitrago, Jennifer Olmas, Kim, Tae Hyun, Rajasekar, Poojitha, Lali, Ferdinand, Kyriakidis, Christos +8 more·2021·Journal of Tissue Engineering
DOI 10.1177/20417314211019238PMC PMC8165536
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Abstract

Current gold standard to treat soft tissue injuries caused by trauma and pathological condition are autografts and off the shelf fillers, but they have inherent weaknesses like donor site morbidity, immuno-compatibility and graft failure. To overcome these limitations, tissue-engineered polymers are seeded with stem cells to improve the potential to restore tissue function. However, their interaction with native tissue is poorly understood so far. To study these interactions and improve outcomes, we have fabricated scaffolds from natural polymers (collagen, fibrin and elastin) by custom-designed processes and their material properties such as surface morphology, swelling, wettability and chemical cross-linking ability were characterised. By using 3D scaffolds, we comprehensive assessed survival, proliferation and phenotype of adipose-derived stem cells in vitro. In vivo, scaffolds were seeded with adipose-derived stem cells and implanted in a rodent model, with X-ray microtomography, histology and immunohistochemistry as read-outs. Collagen-based materials showed higher cell adhesion and proliferation in vitro as well as higher adipogenic properties in vivo. In contrast, fibrin demonstrated poor cellular and adipogenesis properties but higher angiogenesis. Elastin formed the most porous scaffold, with cells displaying a non-aggregated morphology in vitro while in vivo elastin was the most degraded scaffold. These findings of how polymers present in the natural polymers mimicking ECM and seeded with stem cells affect adipogenesis in vitro and in vivo can open avenues to design 3D grafts for soft tissue repair.

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  • In fibrin scaffolds, pores are distributed in the range of 0-35.86 μm and 35.86-89.65 μm, with a total porosity of 43.28%.

    “In the fibrin scaffold, pores can be distributed in the range of 0–35.86 µm and 35.86–89.65 µm, with a total porosity of 43.28% [65].”

    From Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review (2023) · cites this paper as [65]

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Authors
Sawadkar, P., Mandakhbayar, N., Patel, K. D., Buitrago, J. O., Kim, T. H., Rajasekar, P., Lali, F., Kyriakidis, C., Rahmani, B., Mohanakrishnan, J., Dua, R., Greco, K., Lee, J.-H., Kim, H.-W., Knowles, J., & García – Gareta, E.
Affiliations
  • Northwick Park Hospital (GB)
  • Dankook University (KR)
  • University College London (GB)
  • University of Nottingham (GB)
  • Regenerative Biomaterials Group, The RAFT Institute and The Griffin Institute, Northwick Park & Saint Mark’s Hospital, London, UK
Corresponding author
Sawadkar, Prasad
ORCID
Venue
Journal of Tissue Engineering
Publisher
SAGE Publications
Published
2021-01
Volume
12
Pages
20417314211019238-20417314211019238
ISSN
2041-7314 (ISSN-L)
DOI
10.1177/20417314211019238
PMC
PMC8165536
OpenAlex
W3167935525
Cited by
51
References
1
Citations by year
2026: 10 · 2025: 11 · 2024: 11 · 2023: 13 · 2022: 6
FWCI
6.93
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Tissue Engineering (publishedVersion, cc-by-nc)
  • PubMed Central (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
  • Hispana (publishedVersion, cc-by-nc)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • UCL Discovery (University College London) (submittedVersion)
  • ANU Open Research (Australian National University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
Keywords
3D porous scaffolds, collagen, elastin, fibrin, adipose-derived stem cells, adipose tissue regeneration, adipogenesis, angiogenesis, scaffold degradation, extracellular matrix mimics, soft tissue repair

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