Traceability Methods for Cell Line Authentication and Mycoplasma Detection
Open accessAbstract
Many laboratories struggle with mycoplasma contamination and cell line misidentification when growing cells in culture. These well-documented issues affect the scientific research community and have detrimental downstream effects. Research published with suspect cultures can produce misleading results. There is increasing pressure to verify the integrity of experimental and established cell lines before publishing. Therefore, laboratories need to define how and when to perform these critical tests, analyze the results, and determine action plans if disparities exist. Our laboratory is committed to producing cell lines of the highest quality for use in experiments; thus, we created a surveillance strategy for these potential problems. We developed processes for both testing and tracing cell line authentication and mycoplasma detection data. Using these methods, we can protect the integrity of our patient and commercial cell lines, maintaining reliable cultures for our research.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Despite fifty-five years of attention, CCCM persists, with global rates estimated to be between 14% and 46%.
“Despite fifty-five years of attention through reviews, letters, calls to action, surveys, and technical innovations [5][6][7][8], cellular cross-contamination and misidentification persist, with global rates estimated to be between 14% and 46%.”
From The history, landscape, and outlook of human cell line authentication and security (2024) · cites this paper as [5]
The Introduction (page not provided) says cell-line contamination and misidentification remain concerns, and the Results report cross-contamination in six of 69 cell lines tested in the authors’ laboratory. The paper does not give a global rate of 14%–46% or establish the fifty-five-year timeframe, so those parts of the attributed claim are unsupported. (LLM, conf=0.85)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Check your cultures! A list of cross‐contaminated or misidentified cell lines
Capes‐Davis, A., Theodosopoulos, G., Atkin, I., Drexler, H. G., Kohara, A., MacLeod, R. A. F., Masters, J. R., Nakamura, Y., Reid, Y. A., Reddel, R. R., et al. (2010) · International Journal of Cancer · doi:10.1002/ijc.25242 · Cited by 520 · see citing works
Cancer cell line identification by short tandem repeat profiling: power and limitations
Parson, W., Kirchebner, R., Mühlmann, R., Renner, K., Kofler, A., Schmidt, S., & Kofler, R. (2005) · The FASEB Journal · doi:10.1096/fj.04-3062fje · Cited by 129 · see citing works
Investigation of Cross-Contamination and Misidentification of 278 Widely Used Tumor Cell Lines
Huang, Y., Liu, Y., Zheng, C., & Shen, C. (2017) · PLOS ONE · doi:10.1371/journal.pone.0170384 · Cited by 105 · see citing works
Performance of PCR-based and Bioluminescent assays for mycoplasma detection
Falagan-Lotsch, P., Lopes, T. S., Ferreira, N., Balthazar, N., Monteiro, A. M., Borojevic, R., & Granjeiro, J. M. (2015) · Journal of Microbiological Methods · doi:10.1016/j.mimet.2015.08.010 · Cited by 27 · see citing works
Flagged first, then by citations
Research works printed but not linked: 12
- Ji, Y.; Karbaschi, M.; Cooke, M. S. Mycoplasma Infection of Cultured Cells Induces Oxidative Stress and Attenuates Cellular Base Excision Repair Activity. Mutat. Res. 2019, 845, 403054.
- Nikfarjam, L.; Farzaneh, P. Prevention and Detection of Mycoplasma Contamination in Cell Culture. Cell J. 2012, 13, 203-212.
- Bielanski, A.; Vajta, G. Risk of Contamination of Germplasm during Cryopreservation and Cryobanking in IVF Units. Hum. Reprod. 2009, 24, 2457-2467.
- Bajerski, F.; Bürger, A.; Glasmacher, B. Factors Determining Microbial Colonization of Liquid Nitrogen Storage Tanks Used for Archiving Biological Samples. Appl. Microbiol. Biotechnol. 2020, 104, 131-144.
- Uphoff, C. C.; Denkmann, S.-A.; Drexler, H. G. Treatment of Mycoplasma Contamination in Cell Cultures with Plasmocin. Biomed Res. Int. 2012, 2012, 267678.
- Reid, Y.; de Mars, M.; Tian, F. STR Profiling: Authentication of Human Cell Lines and Beyond: Short Tandem Repeat Profiling Is the Most Dictated Method to Confirm Cell Line Identity and Avoid Misidentified or Cross-Contaminated Cell Lines. Biopharm. Int. 2017, 30, 37-41.
- Fusenig, N. E.; Capes-Davis, A.; Bianchini, F.; et al. The Need for a Worldwide Consensus for Cell Line Authentication: Experience Implementing a Mandatory Requirement at the International Journal of Cancer. PLoS Biol. 2017, 15, e2001438.
- Hughes, P.; Marshall, D.; Reid, Y.; et al. The Costs of Using Unauthenticated, Over-Passaged Cell Lines: How Much More Data Do We Need? Biotechniques 2007, 43, 575-586.
- McGrory, J.; Sloots, J.; Hunt, D.; et al. Validation of the Promega Powerplex ® 16 STR Multiplex System for Forensic Casework. Can. Soc. Forensic Sci. J. 2005, 38, 85-99.
- Ensenberger, M. G.; Thompson, J.; Hill, B.; et al. Developmental Validation of the PowerPlex ® 16 HS System: An Improved 16-Locus Fluorescent STR Multiplex. Forensic Sci. Int. Genet. 2010, 4, 257-264.
- Bruford, E. A.; Braschi, B.; Denny, P.; et al. Guidelines for Human Gene Nomenclature. Nat. Genet. 2020, 52, 754-758.
- Dennert, K.; Friedrich, L.; Kumar, R. Creating an Affordable, User-Friendly Electronic Inventory System for Lab Samples. SLAS Technol. 2021, 26, 300-310.
Cited by
Sorted by citations
The history, landscape, and outlook of human cell line authentication and security
Harbut, E., Makris, Y., Pertsemlidis, A., & Bleris, L. (2024) · SLAS Discovery · doi:10.1016/j.slasd.2024.100194 · Cited by 8 · see citing works
- Authors
- Dennert, K., & Kumar, R.
- Affiliations
- Aurora Sinai Medical Center (US)
- Corresponding author
- Kate Dennert
- Venue
- SLAS TECHNOLOGY
- Publisher
- Elsevier BV
- Published
- 2021-12
- Volume
- 26
- Issue
- 6
- Pages
- 630-636
- ISSN
- 2472-6303 (ISSN-L)
- OpenAlex
- W3186119811
- Cited by
- 8
- References
- 4
- Citations by year
- 2025: 1 · 2024: 3 · 2023: 3 · 2022: 1
- FWCI
- 1.24
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- SLAS TECHNOLOGY (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Computer Science › Information Systems › Research Data Management Practices
- Keywords
- cell line authentication, mycoplasma detection, mycoplasma contamination, cell line misidentification, traceability, cell culture quality control
- MeSH terms
- Cell Line Authentication, Cell Line, Humans, Laboratories, Mycoplasma
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
