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Ultrastructural localization of intracellular antigens by the use of protein A-gold complex.

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Roth, J, Bendayan, M, Orci, L·1978·Journal of Histochemistry & Cytochemistry

Abstract

An immunocytochemical technique for the demonstration of intracellular antigens (secretory proteins) on thin sections is reported. Staphylococcal protein A which reacts with the Fc fragment of IgG molecules was labeled with colloidal gold as a marker. The antigenic sites were visualized on aldehyde-fixed and Epon-embedded tissue in a two step procedure. The specific antisera were applied to thin sections for binding to the antigens and then visualized by the protein A-gold complex. By using this technique different secretory proteins of the exocrine and endocrine pancreas were localized. The protein A-gold technique is proposed as a general method for visualization of antigenic sites on thin sections.

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Metadata

Authors
Roth, J., Bendayan, M., & Orci, L.
Venue
Journal of Histochemistry & Cytochemistry
Published
1978-12
Volume
26
Issue
12
Pages
1074-1081
ISSN
  • 0022-1554 (ISSN-L)
  • 1551-5044
OpenAlex
W2064339178
Cited by
980
References
0
FWCI
22.26
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. https://app.cochat.ai/papers/10.1177/26.12.366014
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Roth, J., Bendayan, M., & Orci, L. (1978). Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/26.12.366014

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.