The DOI matches its Crossref record.
A novel immunocytochemical model system for specificity and sensitivity screening of antisera against multiple antigens.
Abstract
A novel, convenient immunocytochemical model system that allows simultaneous testing of multiple peptides and peptide fragments is presented. Screening of antisera for specificity and sensitivity and ready detection of multiple antibody subpopulations is possible with this system. Using the peroxidase-antiperoxidase method, a threshold sensitivity of 100 pg of peptide was attained with the best antisera available. Routine screenings of antisera employ amounts of peptides corresponding to 1.8--180 pmol--concentrations about a thousandfold lower than those used for absorption controls. Because of the minute amounts of antigens needed, specificity screening against rare or costly antigens now becomes feasible.
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Cited by
Sesack, Sr., Aoki, C., & Pickel, V. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-01-00088.1994 · Cited by 461
Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain
Bittar, P. G., Charnay, Y., Pellerin, L., Bouras, C., & Magistretti, P. J. (1996) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1097/00004647-199611000-00001 · Cited by 423
Teitelman, G., Alpert, S., Polak, J. M., Martinez, A., & Hanahan, D. (1993) · Development · doi:10.1242/dev.118.4.1031 · Cited by 368
Metadata
- Authors
- Larsson, L. I.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1981-03-01
- Volume
- 29
- Issue
- 3
- Pages
- 408-410
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2167309585
- Cited by
- 213
- References
- 0
- FWCI
- 6.97
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A novel immunocytochemical model system for specificity and sensitivity screening of antisera against multiple antigens. https://app.cochat.ai/papers/10.1177/29.3.7016977
- The paper
- Larsson, L. I. (1981). A novel immunocytochemical model system for specificity and sensitivity screening of antisera against multiple antigens. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/29.3.7016977
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
