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Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique.
Abstract
The protein A-gold immunocytochemical technique has been modified to allow labeling of cellular antigenic sites on osmium-fixed or postfixed tissues. Several strong oxidizing agents have been found able to restore protein antigenicity on osmicated tissue thin sections. According to the fine structural preservation and intensities of labeling, pretreatment with sodium metaperiodate gave optimal results. Pancreatic secretory proteins (and/or proproteins) as well as insulin (and/or proinsulin) were localized over perfectly preserved rough endoplasmic reticulum (rER), Golgi apparatus, and secretory granules of the corresponding pancreatic cells; carbamyl phosphate synthetase and catalase were revealed over liver mitochondria and peroxisomes, respectively. In addition to the higher resolution in the labeling obtained using osmium-fixed tissues, the present modification confers an additional advantage to the protein A-gold technique by allowing labeling on tissues processed for routine electron microscopy.
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Citations
Cited by
Glucagon‐like peptide‐1 cells in the gastrointestinal tract and pancreas of rat, pig and man
EISSELE, R., GÖKE, R., WILLEMER, S., HARTHUS, H. ‐., VERMEER, H., ARNOLD, R., & GÖKE, B. (1992) · European Journal of Clinical Investigation · doi:10.1111/j.1365-2362.1992.tb01464.x · Cited by 654
De Biasi, S., & Rustioni, A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.20.7820 · Cited by 577
Architecture of ribonucleoprotein complexes in influenza A virus particles
Noda, T., Sagara, H., Yen, A., Takada, A., Kida, H., Cheng, R. H., & Kawaoka, Y. (2006) · Nature · doi:10.1038/nature04378 · Cited by 431
Somogyi, P., Hodgson, A. J., Chubb, I. W., Penke, B., & Erdei, A. (1985) · Journal of Histochemistry & Cytochemistry · doi:10.1177/33.3.2579123 · Cited by 290
Effect of tissue processing on colloidal gold cytochemistry.
Bendayan, M., Nanci, A., & Kan, F. W. (1987) · Journal of Histochemistry & Cytochemistry · doi:10.1177/35.9.3302022 · Cited by 287
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Metadata
- Authors
- Bendayan, M., & Zollinger, M.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1983-01
- Volume
- 31
- Issue
- 1
- Pages
- 101-109
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2065865631
- Cited by
- 633
- References
- 0
- FWCI
- 31.12
- Type
- journal article
- Language
- en
- Open access
- closed
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- Bendayan, M., & Zollinger, M. (1983). Ultrastructural localization of antigenic sites on osmium-fixed tissues applying the protein A-gold technique. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/31.1.6187796
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