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A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling.
Abstract
A large gold cluster (Au1.4nm) was covalently coupled to IgG and Fab' fragments. Its gold core is 1.4 nm in diameter and the Fab'-Au1.4nm immunoconjugate is the smallest gold immunoprobe that can be seen directly in the conventional electron microscope. It is useful in high-resolution immunolabeling, providing a resolution of 7.0 nm. The cluster's visibility can be enhanced with silver development for use in EM or light microscopy for histological purposes, or to detect less than or equal to 0.2 pg of antigen in immunoblots. By using a gold compound with covalent attachment, a number of advantages over colloidal gold probes are realized, including better resolution, stability, uniformity, sensitivity, and complete absence of aggregation; its small size should also improve penetration and more quantitative labeling of antigenic sites.
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Cited by
Photophysical, Photochemical and Photocatalytic Aspects of Metal Nanoparticles
Kamat, P. V. (2002) · The Journal of Physical Chemistry B · doi:10.1021/jp0209289 · Cited by 1,924
Brandner, J. M., Kief, S., Grund, C., Rendl, M., Houdek, P., Kuhn, C., Tschachler, E., Franke, W. W., & Moll, I. (2002) · European Journal of Cell Biology · doi:10.1078/0171-9335-00244 · Cited by 289
Metadata
- Authors
- Hainfeld, J. F., & Furuya, F. R.
- Venue
- Journal of Histochemistry & Cytochemistry
- Publisher
- SAGE Publications
- Published
- 1992-02
- Volume
- 40
- Issue
- 2
- Pages
- 177-184
- ISSN
- 0022-1554 (ISSN-L)
- 1551-5044
- OpenAlex
- W2005649237
- Cited by
- 231
- References
- 0
- FWCI
- 8.47
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. https://app.cochat.ai/papers/10.1177/40.2.1552162
- The paper
- Hainfeld, J. F., & Furuya, F. R. (1992). A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. Journal of Histochemistry & Cytochemistry. https://doi.org/10.1177/40.2.1552162
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
