The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells
Abstract
Human CD133 (AC133)+CD34+ stem and progenitor cells derived from fetal liver and from bone marrow and blood incorporate a functional population of circulating endothelial precursor cells. Vascular endothelial growth factor receptor 3 (VEGFR-3) regulates cardiovascular development and physiological and pathological lymphangiogenesis and angiogenesis. However, the origin of VEGFR-3+ endothelial cells (ECs) and the mechanisms by which these cells contribute to postnatal physiological processes are not known, and the possible existence of VEGFR-3+ lymphatic or vascular EC progenitors has not been studied. Using monoclonal antibodies to the extracellular domain of VEGFR-3, we show that 11% ± 1% of CD34+ cells isolated from human fetal liver, 1.9% ± 0.8% CD34+ cells from human cord blood, and 0.2% ± 0.1% of CD34+ cells from healthy adult blood donors are positive for VEGFR-3. CD34+VEGFR-3+ cells from fetal liver coexpress the stem/precursor cell marker CD133 (AC133). Because mature ECs do not express CD133, coexpression of VEGFR-3 and CD133 on CD34+cells identifies a unique population of stem and progenitor cells. Incubation of isolated CD34+VEGFR-3+ cells in EC growth medium resulted in a strong proliferation (40-fold in 2 weeks) of nonadherent VEGFR-3+ cells. Plating of these cells resulted in the formation of adherent VEGFR-3+Ac-LDL+ (Ac-LDL = acetylated low-density lipoprotein) EC monolayers expressing various vascular and lymphatic endothelial-specific surface markers, including CD34, VE-cadherin, CD51/61, CD105, LYVE-1, and podoplanin. These data demonstrate that human CD34+CD133+ cells expressing VEGFR-3 constitute a phenotypically and functionally distinct population of endothelial stem and precursor cells that may play a role in postnatal lymphangiogenesis and/or angiogenesis.
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Citations
Cites
Isolation of Putative Progenitor Endothelial Cells for Angiogenesis
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AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells
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Induction of tumor lymphangiogenesis by VEGF-C promotes breast cancer metastasis
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Self-citationEvidence for Circulating Bone Marrow-Derived Endothelial Cells
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Self-citationLYVE-1, a New Homologue of the CD44 Glycoprotein, Is a Lymph-specific Receptor for Hyaluronan
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Cited by
Lineage tracing demonstrates the venous origin of the mammalian lymphatic vasculature
Srinivasan, R. S., Dillard, M. E., Lagutin, O. V., Lin, F.-J., Tsai, S., Tsai, M.-J., Samokhvalov, I. M., & Oliver, G. (2007) · Genes & Development · doi:10.1101/gad.1588407 · Cited by 579
The Lymphatic System in Health and Disease
Cueni, L. N., & Detmar, M. (2008) · Lymphatic Research and Biology · doi:10.1089/lrb.2008.1008 · Cited by 288
Lohela, M., Heloterä, H., Haiko, P., Dumont, D. J., & Alitalo, K. (2008) · The American Journal of Pathology · doi:10.2353/ajpath.2008.080378 · Cited by 73
Metadata
- Authors
- Salven, P., Mustjoki, S., Alitalo, R., Alitalo, K., & Rafii, S.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2003-01-01
- Volume
- 101
- Issue
- 1
- Pages
- 168-172
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2001561950
- Cited by
- 377
- References
- 51
- FWCI
- 9.05
- Type
- journal article
- Language
- en
- Open access
- closed
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- Salven, P., Mustjoki, S., Alitalo, R., Alitalo, K., & Rafii, S. (2003). VEGFR-3 and CD133 identify a population of CD34+ lymphatic/vascular endothelial precursor cells. Blood. https://doi.org/10.1182/blood-2002-03-0755
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