The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI
Abstract
Recently, there have been several reports using various superparamagnetic iron oxide (SPIO) nanoparticles to label mammalian cells for monitoring their temporal and spatial migration in vivo by magnetic resonance imaging (MRI). The purpose of this study was to evaluate the efficiency and toxicity of labeling cells using 2 commercially available Food and Drug Administration (FDA)-approved agents, ferumoxides, a suspension of dextran-coated SPIO used as an MRI contrast agent, and protamine sulfate, conventionally used to reverse heparin anticoagulation but also used ex vivo as a cationic transfection agent. After labeling of human mesenchymal stem cells (MSCs) and hematopoietic (CD34+) stem cells and other mammalian cells with ferumoxides-protamine sulfate complexes (FE-Pro), cellular toxicity, functional capacity, and quantitative cellular iron incorporation were determined. FE-Pro-labeled cells demonstrated no short- or long-term toxicity, changes in differentiation capacity of the stem cells, or changes in phenotype when compared with unlabeled cells. Efficient labeling with FE-Pro was observed with iron content per cell varying between 2.01 ± 0.1 pg for CD34+ cells and 10.94 ± 1.86 pg for MSCs with 100% of cells labeled. Cell labeling using these agents should facilitate the translation of this method to clinical trials for evaluation of trafficking of infused or transplanted cells by MRI. (Blood. 2004;104:1217-1223)
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Citations
Cites
Iron oxide-enhanced MR imaging of the liver and spleen: review of the first 5 years
Ferrucci, J. T., & Stark, D. D. (1990) · American Journal of Roentgenology · doi:10.2214/ajr.155.5.2120963 · Cited by 279 · cited 1×
“When used for hepatic MRI, the iron is phagocytosed and accumulates in endosomes of Kupffer cells and reticuloendothelial cells ((13)).”
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Cited by
Laurent, S., Forge, D., Port, M., Roch, A., Robic, C., Vander Elst, L., & Muller, R. N. (2008) · Chemical Reviews · doi:10.1021/cr068445e · Cited by 6,852
Chamberlain, G., Fox, J., Ashton, B., & Middleton, J. (2007) · Stem Cells · doi:10.1634/stemcells.2007-0197 · Cited by 2,381
Reddy, L. H., Arias, J. L., Nicolas, J., & Couvreur, P. (2012) · Chemical Reviews · doi:10.1021/cr300068p · Cited by 2,010
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma
Khakoo, A. Y., Pati, S., Anderson, S. A., Reid, W., Elshal, M. F., Rovira, I. I., Nguyen, A. T., Malide, D., Combs, C. A., Hall, G., et al. (2006) · The Journal of Experimental Medicine · doi:10.1084/jem.20051921 · Cited by 747
Self-citationVillaraza, A. J. L., Bumb, A., & Brechbiel, M. W. (2010) · Chemical Reviews · doi:10.1021/cr900232t · Cited by 632
Metadata
- Authors
- Arbab, A. S., Yocum, G. T., Kalish, H., Jordan, E. K., Anderson, S. A., Khakoo, A. Y., Read, E. J., & Frank, J. A.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2004-08-15
- Volume
- 104
- Issue
- 4
- Pages
- 1217-1223
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2035238560
- Cited by
- 529
- References
- 1
- FWCI
- 25.74
- Type
- journal article
- Language
- en
- Open access
- closed
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- Arbab, A. S., Yocum, G. T., Kalish, H., Jordan, E. K., Anderson, S. A., Khakoo, A. Y., Read, E. J., & Frank, J. A. (2004). Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood. https://doi.org/10.1182/blood-2004-02-0655
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