The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors
Open accessAbstract
Flk1, the gene encoding the vascular endothelial growth factor receptor 2 (VEGFR-2), is a well-known marker for vascular and hematopoietic progenitors and is indispensable for normal hematopoiesis and vasculogenesis. Here we show that Flk1 expression in the early mouse embryo marks a broad spectrum of mesodermal progenitors exiting the primitive streak as well as later mesodermal cell types including some cardiomyocytes, portions of the somites, and all extraembryonic mesoderm cells. These findings made use of an Flk1-lacZ knock-in allele in which the neomycin selection cassette was removed, which resulted in full replication of the endogenous expression of Flk1. Targeted deletion of a region in intron 1 that has been proposed to direct endothelial expression produced no alteration in either endothelial or broader mesodermal expression of the Flk1-lacZ allele. Examination of lacZ expression in homozygotes for the Flk1lacZ neo-out allele revealed that lacZ-expressing mesodermal cells persisted in nonvascular regions. Thus, Flk1 expression marks progenitors with broad mesodermal potential but is not absolutely required for the development of all mesodermal lineages in which it is expressed.
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1 retracted
RETRACTED ARTICLE: Pluripotency of mesenchymal stem cells derived from adult marrow
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Self-citationHeterozygous embryonic lethality induced by targeted inactivation of the VEGF gene
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Self-citationA common precursor for hematopoietic and endothelial cells
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Flk1-positive cells derived from embryonic stem cells serve as vascular progenitors
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A Requirement for Flk1 in Primitive and Definitive Hematopoiesis and Vasculogenesis
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Cited by
Kattman, S. J., Witty, A. D., Gagliardi, M., Dubois, N. C., Niapour, M., Hotta, A., Ellis, J., & Keller, G. (2011) · Cell Stem Cell · doi:10.1016/j.stem.2010.12.008 · Cited by 1,159
Inductive angiocrine signals from sinusoidal endothelium are required for liver regeneration
Ding, B.-S., Nolan, D. J., Butler, J. M., James, D., Babazadeh, A. O., Rosenwaks, Z., Mittal, V., Kobayashi, H., Shido, K., Lyden, D., et al. (2010) · Nature · doi:10.1038/nature09493 · Cited by 836
Ontogeny of the Hematopoietic System
Cumano, A., & Godin, I. (2007) · Annual Review of Immunology · doi:10.1146/annurev.immunol.25.022106.141538 · Cited by 434
Nostro, M. C., Cheng, X., Keller, G. M., & Gadue, P. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2007.10.011 · Cited by 318
Directed differentiation of human embryonic stem cells toward chondrocytes
Oldershaw, R. A., Baxter, M. A., Lowe, E. T., Bates, N., Grady, L. M., Soncin, F., Brison, D. R., Hardingham, T. E., & Kimber, S. J. (2010) · Nature Biotechnology · doi:10.1038/nbt.1683 · Cited by 302
Metadata
- Authors
- Ema, M., Takahashi, S., & Rossant, J.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-01-01
- Volume
- 107
- Issue
- 1
- Pages
- 111-117
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2081794739
- Cited by
- 295
- References
- 31
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors. https://app.cochat.ai/papers/10.1182/blood-2005-05-1970
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- Ema, M., Takahashi, S., & Rossant, J. (2006). Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors. Blood. https://doi.org/10.1182/blood-2005-05-1970
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