The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Pf4-Cre transgenic mice allow the generation of lineage-restricted gene knockouts for studying megakaryocyte and platelet function in vivo
Abstract
To generate transgenic mice that express Cre-recombinase exclusively in the megakaryocytic lineage, we modified a mouse bacterial artificial chromosome (BAC) clone by homologous recombination and replaced the first exon of the platelet factor 4 (Pf4), also called CXCL4, with a codon-improved Cre cDNA. Several strains expressing the transgene were obtained and one strain, Q3, was studied in detail. Crossing Q3 mice with the ROSA26-lacZ reporter strain showed that Cre-recombinase activity was confined to megakaryocytes. These results were further verified by crossing the Q3 mice with a strain containing loxP-flanked integrin β1. Excision of this conditional allele in megakaryocytes was complete at the DNA level, and platelets were virtually devoid of the integrin β1 protein. The Pf4-Cre transgenic strain will be a valuable tool to study megakaryopoiesis, platelet formation, and platelet function.
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Malehmir, M., Pfister, D., Gallage, S., Szydlowska, M., Inverso, D., Kotsiliti, E., Leone, V., Peiseler, M., Surewaard, B. G. J., Rath, D., et al. (2019) · Nature Medicine · doi:10.1038/s41591-019-0379-5 · Cited by 398
Metadata
- Authors
- Tiedt, R., Schomber, T., Hao-Shen, H., & Skoda, R. C.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-10-10
- Volume
- 109
- Issue
- 4
- Pages
- 1503-1506
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1996530649
- Cited by
- 422
- References
- 21
- FWCI
- 4.14
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Pf4-Cre transgenic mice allow the generation of lineage-restricted gene knockouts for studying megakaryocyte and platelet function in vivo. https://app.cochat.ai/papers/10.1182/blood-2006-04-020362
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- Tiedt, R., Schomber, T., Hao-Shen, H., & Skoda, R. C. (2007). Pf4-Cre transgenic mice allow the generation of lineage-restricted gene knockouts for studying megakaryocyte and platelet function in vivo. Blood. https://doi.org/10.1182/blood-2006-04-020362
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