The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.
Peroxiredoxin 2 functions as a noncatalytic scavenger of low-level hydrogen peroxide in the erythrocyte
Abstract
Peroxiredoxin 2 (Prx2), a thiol-dependent peroxidase, is the third most abundant protein in the erythrocyte, and its absence in knock-out mice gives rise to hemolytic anemia. We have found that in human erythrocytes, Prx2 was extremely sensitive to oxidation by H2O2, as dimerization was observed after exposure of 5 × 106 cells/mL to 0.5 μM H2O2. In contrast to Prx2 in Jurkat T lymphocytes, Prx2 was resistant to overoxidation (oxidation of the cysteine thiol to a sulfinic/sulfonic acid) in erythrocytes. Reduction of dimerized Prx2 in the erythrocyte occurred very slowly, with reversal occurring gradually over a 20-minute period. Very low thioredoxin reductase activity was detected in hemolysates. We postulate that this limits the rate of Prx2 regeneration, and this inefficiency in recycling prevents the overoxidation of Prx2. We also found that Prx2 was oxidized by endogenously generated H2O2, which was mainly derived from hemoglobin autoxidation. Our results demonstrate that in the erythrocyte Prx2 is extremely efficient at scavenging H2O2 noncatalytically. Although it does not act as a classical antioxidant enzyme, its high concentration and substrate sensitivity enable it to handle low H2O2 concentrations efficiently. These unique redox properties may account for its nonredundant role in erythrocyte defense against oxidative stress.
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Cited by
Thiol chemistry and specificity in redox signaling
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Self-citationSignaling Functions of Reactive Oxygen Species
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Focus on mammalian thioredoxin reductases — Important selenoproteins with versatile functions
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Paradoxical Roles of Antioxidant Enzymes: Basic Mechanisms and Health Implications
Lei, X. G., Zhu, J.-H., Cheng, W.-H., Bao, Y., Ho, Y.-S., Reddi, A. R., Holmgren, A., & Arnér, E. S. J. (2016) · Physiological Reviews · doi:10.1152/physrev.00010.2014 · Cited by 429
Metadata
- Authors
- Low, F. M., Hampton, M. B., Peskin, A. V., & Winterbourn, C. C.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2006-11-14
- Volume
- 109
- Issue
- 6
- Pages
- 2611-2617
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1975984844
- Cited by
- 282
- References
- 45
- FWCI
- 5.28
- Type
- journal article
- Language
- en
- Open access
- closed
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- Low, F. M., Hampton, M. B., Peskin, A. V., & Winterbourn, C. C. (2007). Peroxiredoxin 2 functions as a noncatalytic scavenger of low-level hydrogen peroxide in the erythrocyte. Blood. https://doi.org/10.1182/blood-2006-09-048728
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