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Cited by 78Hematopoietic Stem Cell TransplantationKruppel-like factors researchAcute Myeloid Leukemia Research

Runx1 is involved in primitive erythropoiesis in the mouse

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Yokomizo, Tomomasa, Hasegawa, Kazuteru, Ishitobi, Hiroyuki, Osato, Motomi, Ema, Masatsugu, Ito, Yoshiaki, Yamamoto, Masayuki, Takahashi, Satoru·2008·Blood
DOI 10.1182/blood-2007-05-091637
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Abstract

Targeted disruption of the Runx1/ AML1 gene in mice has demonstrated that it is required for the emergence of definitive hematopoietic cells but that it is not essential for the formation of primitive erythrocytes. These findings led to the conclusion that Runx1 is a stage-specific transcription factor acting only during definitive hematopoiesis. However, the zebrafish and Xenopus homologs of Runx1 have been shown to play roles in primitive hematopoiesis, suggesting that mouse Runx1 might also be involved in the development of primitive lineages. In this study, we show that primitive erythrocytes in Runx1(-/-) mice display abnormal morphology and reduced expression of Ter119, Erythroid Kruppel-like factor (EKLF, KLF1), and GATA-1. These results suggest that mouse Runx1 plays a role in the development of both primitive and definitive hematopoietic cells.

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  • Emergence of hematopoietic progenitors from the hemangioblast coincides with the activation of transcription factors required for hematopoiesis, such as Runx1 and Gata1.

    “Emergence of hematopoietic progenitors from the hemangioblast coincides with the activation of transcription factors required for hematopoiesis, such as Runx1 and Gata1”

    From Single-Cell Transcriptomics Reveals Early Emergence of Liver Parenchymal and Non-parenchymal Cell Lineages (2020) · cites this paper as Yokomizo et al., 2008

    The Results section ('Reduced expression of EKLF and GATA-1 in Runx1 -/ embryos'; page not provided) identifies GATA-1 as important for erythrocyte differentiation and reports reduced GATA-1 expression in Runx1-deficient cells. 'Transgenic rescue of Runx1 -/ primitive erythrocytes' says GATA-1 is expressed in early erythroid progenitors. The paper does not establish that activation of both factors coincides with progenitors emerging from the hemangioblast. (LLM, conf=0.82)

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References 41 · Cited by 78

Research works printed but not linked: 34

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Authors
Yokomizo, T., Hasegawa, K., Ishitobi, H., Osato, M., Ema, M., Ito, Y., Yamamoto, M., & Takahashi, S.
Affiliations
  • University of Tsukuba (JP)
  • Institute of Molecular and Cell Biology (SG)
ORCID
Venue
Blood
Publisher
American Society of Hematology
Published
2008-04-15
Volume
111
Issue
8
Pages
4075-4080
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
DOI
10.1182/blood-2007-05-091637
OpenAlex
W1996300461
Cited by
78
References
41
Citations by year
2026: 2 · 2025: 3 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 6 · 2017: 5 · 2016: 5 · 2015: 3 · 2014: 4 · 2013: 9 · 2012: 3
FWCI
4.79
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed (publishedVersion)
  • Institutional Repositories DataBase (IRDB) (acceptedVersion)
  • Terrestrial Environment Research Center (University of Tsukuba) (submittedVersion, cc-by-nc-nd)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Kruppel-like factors research
  • Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
Keywords
RUNX1, primitive erythropoiesis, definitive hematopoiesis, erythrocyte morphology, Ter119, EKLF, KLF1, GATA-1, hematopoietic development
MeSH terms
Animals, Cell Differentiation, Embryo, Mammalian, Erythrocytes, Erythropoiesis, Mice, Inbred C57BL, Mice, Transgenic, RNA, Yolk Sac, Down-Regulation, Erythroid Precursor Cells, Oligonucleotide Array Sequence Analysis, Core Binding Factor Alpha 2 Subunit, GATA1 Transcription Factor, Mice, Kruppel-Like Transcription Factors

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