Critical Role of Transcription Factor PU.1 in the Expression of CD80 and CD86 on Dendritic Cells
Abstract
In this study, we investigated the role of a transcription factor, PU.1, in the regulation of CD80 and CD86 expression in dendritic cells (DCs). A chromatin immunoprecipitation assay revealed that PU.1 is constitutively bound to the CD80 and CD86 promoters in bone marrow-derived DCs. In addition, co-expression of PU.1 resulted in the transactivation of the CD80 and CD86 promoters in a reporter assay. The binding of PU.1 to cis-enhancing regions was confirmed by electromobility gel-shift assay. As expected, inhibition of PU.1 expression by short interfering RNA (siRNA) in bone marrow-derived DCs resulted in marked down-regulation of CD80 and CD86 expression. Moreover, overexpression of PU.1 in murine bone marrow-derived lineage-negative cells induced the expression of CD80 and CD86 in the absence of monocyte/DC-related growth factors and/or cytokines. Based on these results, we conclude that PU.1 is a critical factor for the expression of CD80 and CD86. We also found that subcutaneous injection of PU.1 siRNA or topical application of a cream-emulsified PU.1 siRNA efficiently inhibited murine contact hypersensitivity. Our results suggest that PU.1 is a potential target for the treatment of immune-related diseases.
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PU.1 functions as an immune activator by inducing the expression of IL-9 and CD80.
“PU.1 also functions as an immune activator by inducing the expression of IL‐9 and CD80 [58, 59, 60].”
From Targeting Microglial Transcriptional Reprogramming as a Therapy Strategy for Alzheimer's Disease (2026) · cites this paper as [59]
Cites
PU. 1 is required for myeloid-derived but not lymphoid-derived dendritic cells
Guerriero, A., Langmuir, P. B., Spain, L. M., & Scott, E. W. (2000) · Blood · doi:10.1182/blood.v95.3.879.003k13_879_885 · Cited by 190 · see citing works
Wang, I.-M., Contursi, C., Masumi, A., Ma, X., Trinchieri, G., & Ozato, K. (2000) · The Journal of Immunology · doi:10.4049/jimmunol.165.1.271 · Cited by 187 · see citing works
Crystal structure of PU. 1/IRF-4/DNA ternary complex
Escalante, C. R., Brass, A. L., Pongubala, J. M. R., Shatova, E., Shen, L., Singh, H., & Aggarwal, A. K. (2002) · Molecular Cell · doi:10.1016/s1097-2765(02)00703-7 · Cited by 154 · see citing works
Immune cell-specific amplification of interferon signaling by the IRF-4/8-PU. 1 complex
Kanno, Y., Levi, B.-Z., Tamura, T., & Ozato, K. (2005) · Journal of Interferon & Cytokine Research · doi:10.1089/jir.2005.25.770 · Cited by 128 · see citing works
Functional analysis of PU.1 domains in monocyte‐specific gene regulation
Nishiyama, C., Nishiyama, M., Ito, T., Masaki, S., Masuoka, N., Yamane, H., Kitamura, T., Ogawa, H., & Okumura, K. (2004) · FEBS Letters · doi:10.1016/s0014-5793(04)00116-4 · Cited by 34 · see citing works
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Transcription factor PU.1 and immune cell differentiation (Review)
Li, G., Hao, W., & Hu, W. (2020) · International Journal of Molecular Medicine · doi:10.3892/ijmm.2020.4763 · Cited by 98 · see citing works
Kitamura, N., Yokoyama, H., Yashiro, T., Nakano, N., Nishiyama, M., Kanada, S., Fukai, T., Hara, M., Ikeda, S., Ogawa, H., et al. (2012) · Journal of Allergy and Clinical Immunology · doi:10.1016/j.jaci.2011.10.019 · Cited by 55 · see citing works
Self-citationA transcription factor PU.1 is critical for Ccl22 gene expression in dendritic cells and macrophages
Yashiro, T., Nakano, S., Nomura, K., Uchida, Y., Kasakura, K., & Nishiyama, C. (2019) · Scientific Reports · doi:10.1038/s41598-018-37894-9 · Cited by 29 · see citing works
Self-citationTargeting Microglial Transcriptional Reprogramming as a Therapy Strategy for Alzheimer's Disease
Kim, B., Wang, S. S., Kim, J. R., & Kim, J. (2026) · BioEssays · doi:10.1002/bies.70186
- Authors
- Kanada, S., Nishiyama, C., Nakano, N., Suzuki, R., Maeda, K., Hara, M., Kitamura, N., Ogawa, H., & Okumura, K.
- Affiliations
- Juntendo University (JP)
- Atopy (Allergy) Research Center and
- Corresponding author
- Kanada, Shunsuke
- Nishiyama, Chiharu
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 2011-02-17
- Volume
- 117
- Issue
- 7
- Pages
- 2211-2222
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2041778457
- Cited by
- 73
- References
- 5
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 12 · 2023: 9 · 2022: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 6
- FWCI
- 1.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Japan Society for the Promotion of Science (23390260)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Keywords
- PU.1, CD80, CD86, dendritic cells, transcriptional regulation, promoter binding, bone marrow-derived dendritic cells, contact hypersensitivity, siRNA knockdown
- MeSH terms
- Proto-Oncogene Protein Spi-1, Administration, Topical, Animals, Base Sequence, Binding Sites, Dendritic Cells, Dermatitis, Contact, Female, Mice, Inbred BALB C, Molecular Sequence Data, Promoter Regions, Genetic, Proto-Oncogene Proteins, Transcriptional Activation, Trans-Activators, Down-Regulation, DNA, Complementary, B7-1 Antigen, Transcription Initiation Site, RNA, Small Interfering, Mice, B7-2 Antigen, In Vitro Techniques
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