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Phosphorylation of the myosin IIA tailpiece regulates single myosin IIA molecule association with lytic granules to promote NK-cell cytotoxicity

Sanborn, Keri B., Mace, Emily M., Rak, Gregory D., Difeo, Analisa, Martignetti, John A., Pecci, Alessandro, Bussel, James B., Favier, Rémi, Orange, Jordan S.·2011·Blood
DOI 10.1182/blood-2011-03-344846
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Abstract

Natural killer (NK) cells are innate immune lymphocytes that provide critical defense against virally infected and transformed cells. NK-cell cytotoxicity requires the formation of an F-actin rich immunologic synapse (IS), as well as the polarization of perforin-containing lytic granules to the IS and secretion of their contents at the IS. It was reported previously that NK-cell cytotoxicity requires nonmuscle myosin IIA function and that granule-associated myosin IIA mediates the interaction of granules with F-actin at the IS. In the present study, we evaluate the nature of the association of myosin IIA with lytic granules. Using NK cells from patients with mutations in myosin IIA, we found that the nonhelical tailpiece is required for NK-cell cytotoxicity and for the phosphorylation of granule-associated myosin IIA. Ultra-resolution imaging techniques demonstrated that single myosin IIA molecules associate with NK-cell lytic granules via the nonhelical tailpiece. Phosphorylation of myosin IIA at residue serine 1943 (S1943) in the tailpiece is needed for this linkage. This defines a novel mechanism for myosin II function, in which myosin IIA can act as a single-molecule actin motor, claiming granules as cargo through tail-dependent phosphorylation for the execution of a pre-final step in human NK-cell cytotoxicity.

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Cited by

Metadata

Authors
Sanborn, K. B., Mace, E. M., Rak, G. D., Difeo, A., Martignetti, J. A., Pecci, A., Bussel, J. B., Favier, R., & Orange, J. S.
Affiliations
  • Immunology Graduate Group, University of Pennsylvania School of Medicine, Philadelphia, PA;
  • Children's Hospital Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA;
  • Department of Genetics and Genomic Sciences, Mount Sinai School of Medicine, New York, NY;
  • Department of Internal Medicine, Istituto di Ricovero e Cura a Carattere Scientifico San Matteo, University of Pavia, Pavia, Italy;
  • Weill-Cornell Medical College of Cornell University, New York, NY; and
  • Assistance Publique–Hopitaux de Paris, Centre de Recherches en Physique des Plasmas, Armand Trousseau Children's Hospital, Villejuif, France
Venue
Blood
Publisher
American Society of Hematology
Published
2011-11-24
Volume
118
Issue
22
Pages
5862-5871
ISSN
  • 0006-4971
  • 1528-0020
DOI
10.1182/blood-2011-03-344846
Cited by
56
References
1
Language
en

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