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Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride
Abstract
A method is described for demonstrating leukocyte peroxidase activity in which benzidine dihydrochloride is used as the indicator compound instead of the more commonly used but potentially more hazardous benzidine base. The method is highly sensitive and rapid and permits the use of fixed blood smears and organ imprints. The incubation mixture, which incorporates safranin as a counterstain, may be used over and over again, for as long as 6 months. The method is also applicable to fresh frozen tissue sections.
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Citations
Cited by
Lee, T. H., Hoover, R. L., Williams, J. D., Sperling, R. I., Ravalese, J., Spur, B. W., Robinson, D. R., Corey, E. J., Lewis, R. A., & Austen, K. F. (1985) · New England Journal of Medicine · doi:10.1056/nejm198505093121903 · Cited by 1,333
IDENTIFICATION OF A NOVEL CELL TYPE IN PERIPHERAL LYMPHOID ORGANS OF MICE
Steinman, R. M., Lustig, D. S., & Cohn, Z. A. (1974) · The Journal of Experimental Medicine · doi:10.1084/jem.139.6.1431 · Cited by 1,050
Establishment of an immature mast cell line from a patient with mast cell leukemia
Butterfield, J. H., Weiler, D., Dewald, G., & Gleich, G. J. (1988) · Leukemia Research · doi:10.1016/0145-2126(88)90050-1 · Cited by 737
Kulessa, H., Frampton, J., & Graf, T. (1995) · Genes & Development · doi:10.1101/gad.9.10.1250 · Cited by 418
Fast, D. J., Schlievert, P. M., & Nelson, R. D. (1989) · Infection and Immunity · doi:10.1128/iai.57.1.291-294.1989 · Cited by 272
Metadata
- Authors
- KAPLOW, L. S., & Ladd, C.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1965-08-01
- Volume
- 26
- Issue
- 2
- Pages
- 215-219
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1729011202
- Cited by
- 653
- References
- 0
- FWCI
- 2.04
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride. https://app.cochat.ai/papers/10.1182/blood.v26.2.215.215
- The paper
- KAPLOW, L. S., & Ladd, C. (1965). Brief Report: Simplified Myeloperoxidase Stain Using Benzidine Dihydrochloride. Blood. https://doi.org/10.1182/blood.v26.2.215.215
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
