The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Direct demonstration of elevated aldehyde dehydrogenase in human hematopoietic progenitor cells
Abstract
Relative levels of cytoplasmic aldehyde dehydrogenase (ALDH) were determined in selected subpopulations of normal human bone marrow cells using a flow cytometric assay that simultaneously detects a cell surface antigen (as a marker of cell lineage and developmental stage) and the level of ALDH. The intracellular level of this enzyme has been shown to be directly related to cellular resistance to activated cyclophosphamide and is believed to be important in the survival of cells capable of repopulating marrow in autologous bone marrow transplant procedures. Western blot analysis and flow cytometric analysis of four murine cell lines with known ALDH levels were used to establish the relation between ALDH content and fluorescence with an affinity-purified anti-mouse ALDH antibody. An affinity purified anti- human ALDH antibody, characterized by immunoblotting of cytosolic extracts of cell lines with known ALDH content, was used to determine relative ALDH levels in the marrow subpopulations. We found that hematopoietic progenitor cells express the highest level of ALDH, while lymphocytes express the lowest level. Immature erythroid cells express ALDH at a level intermediate between progenitor cells and lymphocytes.
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Yeager, A. M., Kaizer, H., Santos, G. W., Saral, R., Colvin, O. M., Stuart, R. K., Braine, H. G., Burke, P. J., Ambinder, R. F., Burns, W. H., et al. (1986) · New England Journal of Medicine · doi:10.1056/nejm198607173150301 · Cited by 426
Self-citationStrauss, L. C., Rowley, S. D., Russa, V. F. L., Sharkis, S. J., Stuart, R. K., & Civin, C. (1986) · PubMed · Cited by 173
Self-citationCharacterization of cytosolic aldehyde dehydrogenase from cyclophosphamide resistant L1210 cells.
Russo, J. E., & Hilton, A. (1988) · PubMed · Cited by 118
Self-citationKastan, M., Stone, K., & Civin, C. (1989) · Blood · doi:10.1182/blood.v74.5.1517.1517 · Cited by 89
Self-citationSládek, N. E., & Landkamer, G. J. (1985) · PubMed · Cited by 83
Purification and partial characterization of aldehyde dehydrogenase from human erythrocytes
Inoue, K., Nishimukai, H., & Yamasawa, K. (1979) · Biochimica et Biophysica Acta (BBA) - Enzymology · doi:10.1016/0005-2744(79)90046-9 · Cited by 71
Cited by
Chaudhary, P. M., & Roninson, I. B. (1991) · Cell · doi:10.1016/0092-8674(91)90141-k · Cited by 934
ALDH1 as a Functional Marker of Cancer Stem and Progenitor Cells
Douville, J., Beaulieu, R., & Balicki, D. (2009) · Stem Cells and Development · doi:10.1089/scd.2008.0055 · Cited by 356
Hess, D. A., Meyerrose, T. E., Wirthlin, L., Craft, T. P., Herrbrich, P. E., Creer, M. H., & Nolta, J. A. (2004) · Blood · doi:10.1182/blood-2004-02-0448 · Cited by 351
Aldehyde Dehydrogenases: Not Just Markers, but Functional Regulators of Stem Cells
Vassalli, G. (2019) · Stem Cells International · doi:10.1155/2019/3904645 · Cited by 300
Aldehyde dehydrogenases and cell proliferation
Muzio, G., Maggiora, M., Paiuzzi, E., Oraldi, M., & Canuto, R. A. (2012) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2011.11.033 · Cited by 297
Metadata
- Authors
- Kastan, M., Schlaffer, E., Russo, J., Colvin, O., Civin, C., & Hilton, J.
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1990-05-15
- Volume
- 75
- Issue
- 10
- Pages
- 1947-1950
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W1495710350
- Cited by
- 307
- References
- 7
- FWCI
- 3.53
- Type
- journal article
- Language
- en
- Open access
- closed
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- Kastan, M., Schlaffer, E., Russo, J., Colvin, O., Civin, C., & Hilton, J. (1990). Direct demonstration of elevated aldehyde dehydrogenase in human hematopoietic progenitor cells. Blood. https://doi.org/10.1182/blood.v75.10.1947.1947
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
