The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Establishment and characterization of cloned human thymic epithelial cell lines—Analysis of adhesion molecule expression and cytokine production
Open accessAbstract
The thymic stromal microenvironment is required for the generation of immunocompetent T lymphocytes. However, the different thymic stromal cell types have not been fully characterized and their roles regarding T-cell development are not completely understood. To address the phenotypic characteristics of the epithelial component of the human thymic microenvironment as well as its functional involvement in T-cell development, we have established cloned thymic epithelial cell (TEC) lines from fetal and postnatal human thymuses by an explant technique, repeated subculture, and limiting dilution cloning. These cloned TEC lines were shown to be derived from cortical epithelium and to express a number of cell-surface molecules including CD40, major histocompatibility complex (MHC) HLA-ABC and HLA-DR antigens, homing- associated cell-adhesion molecule (H-CAM), intercellular adhesion molecule-1 (ICAM-1), leukocyte function-associated antigen 3 (LFA-3), and beta 1 subfamily integrins. Finally, both postnatal and fetal TEC clones were shown to produce interleukin-1 alpha (IL-1 alpha), IL-6, and IL-7. These well-defined cloned TEC lines may provide useful tools for the study of TEC biology and for the understanding of the precise role played by TEC in human T-cell development.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In a 1994 report, the establishment of a group of novel thymic cell lines (F2-4E5, F2-5B6, P1-1A3 and P1-4D6) was announced.
“In a 1994 report, the establishment of a group of novel thymic cell lines (F2-4E5, F2-5B6, P1-1A3 and P1-4D6) [39] was announced.”
From The ghosts of HeLa: How cell line misidentification contaminates the scientific literature (2017) · cites this paper as [39]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Regulation of hemopoiesis by bone marrow stromal cells and their products
Dorshkind, K. (1990) · Annual Review of Immunology · doi:10.1146/annurev.iy.08.040190.000551 · Cited by 504
Flagged first, then by citations
Research works printed but not linked: 2
- Vollger LW, Tuck DT, Springer TA, Haynes BF, Singer KH: Thymocyte binding to human thymic epithelial cells is inhibited by monoclonal antibodies to CD-2 and LFA-3. J Immunol 138:358, 1987
- Benoist C, Mathis D: Regulation of major histocompatibility complex class IT genes: X , Y and other letters of the alphabet. Annu Rev Immunol 8:68 I , I990 40. Springer TA: Adhesion receptors of the immune system. Na- ture 346:425, 1990
Cited by
Sorted by citations
The ghosts of HeLa: How cell line misidentification contaminates the scientific literature
Horbach, S. P. J. M., & Halffman, W. (2017) · PLOS ONE · doi:10.1371/journal.pone.0186281 · Cited by 167
Metadata
- Authors
- Fernandez, E., Vicente, A., Zapata, A., Brera, B., Lozano, J., Martinez, C., & Toribio, M.
- Affiliations
- Centro de Biología Molecular Severo Ochoa (ES)
- Centro de Biologia Molecular Severo Ochoa, Universidad Autonoma de Madrid, Spain.
- ORCID
- Zapata, A
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1994-06-01
- Volume
- 83
- Issue
- 11
- Pages
- 3245-3254
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2270521400
- Cited by
- 84
- References
- 1
- Citations by year
- 2023: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 6 · 2013: 1 · 2012: 1
- FWCI
- 2.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- thymic epithelial cells, thymic stroma, T cell development, adhesion molecules, cytokine production, HLA-ABC, HLA-DR, ICAM-1, LFA-3, beta1 integrin, CD40, fetal thymus, interleukin-1 alpha, interleukin-6, IL-7, thymic microenvironment
- MeSH terms
- Carrier Proteins, Cell Line, Epithelial Cells, HLA-DR Antigens, Humans, Infant, Receptors, Cell Surface, Thymus Gland, Histocompatibility Antigens Class I, Cell Adhesion Molecules, Integrins, Receptors, Lymphocyte Homing, Cytokines, Intercellular Adhesion Molecule-1, Hyaluronan Receptors, Integrin beta1
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
