The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Brain endothelium lack one of two pathways of P-selectin-mediated neutrophil adhesion
Abstract
P-selectin, an endothelial leukocyte adhesion receptor, is rapidly translocated to the cell surface upon release from storage granules called Weibel-Palade bodies and is also transcriptionally upregulated upon cytokine stimulation of endothelial cells (ECs). These two pathways of surface expression are coincident with the rapid and cytokine-inducible pathway of neutrophil adhesion to ECs. Constitutive P-selectin expression is largely absent in cultured murine brain microvascular EC (BMEC) monolayers, but interleukin-1beta and tumor necrosis factor-alpha stimulation for 4 hours leads to dramatic P-selectin upregulation. The functional relevance of differential P-selectin expression in these cells was examined by studying BMECs derived from wild-type mice and P-selectin-deficient mice. We show that P-selectin deficiency does not affect Weibel-Palade body formation or their release in response to short-acting agonists. However, in the absence of P-selectin, the brain endothelium is unable to support neutrophil adhesion after stimulation with these agonists, which may contribute to the immune privilege status of the brain. We show that P- selectin does play a major role in supporting neutrophil adhesion in the cytokine-induced pathway in BMECs in the context of other cytokine- inducible endothelial-leukocyte adhesion receptors, E-selectin, ICAM-1, and VCAM-1.
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In the citing paper
“Under steady state, brain endothelial cells do not support myeloid cell adhesion because of a lack of surface P-selectin expression (79).”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [79]
In the citing paper
“Subarachnoid and pial vessels also restrict extravasation of leukocytes; however, migration across these vessels is more efficient relative to parenchymal vessels (86). This is because meningeal, unlike parenchymal, vascular endothelial cells constitutively express P-selectin during steady state, which is stored in Weibel-Palade bodies, allowing rapid release to the endothelial surface (79, 86).”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [79]
In the citing paper
“In contrast to most peripheral vasculature, blood vessels in the CNS parenchyma lack P-selectin in Weibel-Palade bodies and only express low levels of this protein under steady state (79).”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [79]
In the citing paper
“In fact, constitutive expression of P-selectin is only present in meningeal vessels.”
From The anatomy and immunology of vasculature in the central nervous system (2019) · cites this paper as [79]
Citations
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Ransohoff, R. M., & Engelhardt, B. (2012) · Nature reviews. Immunology · doi:10.1038/nri3265 · Cited by 1,029
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Metadata
- Authors
- Barkalow, F., Goodman, M., Gerritsen, M., & Mayadas, T.
- Affiliations
- Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA 02115, USA.
- Brigham and Women's Hospital (US)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1996-12-15
- Volume
- 88
- Issue
- 12
- Pages
- 4585-4593
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2338797839
- Cited by
- 110
- References
- 42
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2022: 6 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 5 · 2017: 4 · 2016: 2 · 2015: 5 · 2014: 3 · 2013: 3 · 2012: 5
- FWCI
- 1.80
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ns033296-02)
- National Heart, Lung, and Blood Institute (T32HL007627, 5T32HL07627-10)
- National Institute of Neurological Disorders and Stroke (R01-NS33296)
- Topics
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- P-selectin, neutrophil adhesion, brain microvascular endothelial cells, Weibel-Palade bodies, IL-1β, TNF-α, immune privilege, E-selectin, ICAM-1, VCAM-1, leukocyte adhesion molecules
- MeSH terms
- Animals, Brain, Brain Chemistry, Cell Adhesion, Cytoplasmic Granules, Endothelium, Vascular, Microcirculation, Neutrophils, P-Selectin, Mice
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