The DOI matches its Crossref record; 35 of the 37 references it cites were found in a registry or an index.
Regulation of iron metabolism in murine J774 macrophages: role of nitric oxide-dependent and -independent pathways following activation with gamma interferon and lipopolysaccharide.
Abstract
To elucidate the pathways by which nitric oxide (NO) influences macrophage iron metabolism, the uptake, release, and intracellular distribution of iron in the murine macrophage cell line J774 has been investigated, together with transferrin receptor (TfR) expression and iron-regulatory protein (IRP1 and IRP2) activity. Stimulation of macrophages with interferon-γ (IFN-γ) and/or lipopolysaccharide (LPS) decreased Fe uptake from transferrin (Tf), and there was a concomitant downregulation of TfR expression. These effects were mediated by NO-dependent and NO-independent mechanisms. Addition of the NO synthase (NOS) inhibitor N-monomethyl arginine (NMMA) partially restored Fe uptake but either had no effect on or downregulated TfR expression, which suggests that NO by itself is able to affect iron availability. Analysis of the intracellular distribution of incorporated iron revealed that in IFN-γ/LPS-activated macrophages there was a decreased amount and proportion of ferritin-bound iron and a compensatory increase in insoluble iron, which probably consists mainly of iron bound to intracellular organelles. Finally, although NO released by IFN-γ/LPS-activated macrophages increased the iron-responsive element (IRE)-binding activity of both IRP1 and IRP2, IFN-γ treatment decreased IRP2 activity in an NO-independent manner. This study demonstrates that the effect of IFN-γ and/or LPS on macrophage iron metabolism is complex, and is not entirely due to either NO-or to IRP-mediated mechanisms. The overall effect is to decrease iron uptake, but not its utilization.
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In the citing paper
“Second, TNF-α, IL-1, IL-6, and interferon-gamma (IFN-γ) affect macrophage iron homeostasis by different avenues. They increase the uptake of transferrin and non-transferrin bound iron by modulating the expression of transferrin receptor-1 and divalent metal transporter-1, respectively (Byrd and Horwitz, 1993; Fahmy and Young, 1993; Mulero and Brock, 1999; Ludwiczek et al., 2003).”
From Iron at the interface of immunity and infection (2014) · cites this paper as Mulero and Brock, 1999
In the citing paper
“In addition, many of these cytokines as well as the anti-inflammatory cytokines IL-4, IL-10, and IL-13 promote efficient iron storage within macrophages/monocytes by increasing the expression of ferritin, both at the transcriptional and posttranscriptional level (Byrd and Horwitz, 1993; Weiss et al., 1997a; Mulero and Brock, 1999; Arosio and Levi, 2002; Tilg et al., 2002; Ludwiczek et al., 2003).”
From Iron at the interface of immunity and infection (2014) · cites this paper as Mulero and Brock, 1999
Citations
Cites
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Nitric oxide and the regulation of gene expression
Bogdan, C. (2001) · Trends in Cell Biology · doi:10.1016/s0962-8924(00)01900-0 · Cited by 534
Cytokine-mediated regulation of iron transport in human monocytic cells
Ludwiczek, S., Aigner, E., Theurl, I., & Weiss, G. (2003) · Blood · doi:10.1182/blood-2002-08-2459 · Cited by 420
Iron Metabolism in the Reticuloendothelial System
Knutson, M. D., & Wessling‐Resnick, M. (2003) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/713609210 · Cited by 360
Iron at the interface of immunity and infection
Nairz, M., Haschka, D., Demetz, E., & Weiss, G. (2014) · Frontiers in Pharmacology · doi:10.3389/fphar.2014.00152 · Cited by 325
“…promote efficient iron storage within macrophages/monocytes by increasing the expression of ferritin, both at the transcriptional and posttranscriptional level (Byrd and Horwitz, 1993; Weiss et al., 1997a; Mulero and Brock, 1999; Arosio and Levi, 2002; Tilg et al., 2002; Ludwiczek et al., 2003).”
BackgroundIron metabolism in the anemia of chronic disease
Weiss, G. (2009) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2008.08.006 · Cited by 322
Metadata
- Authors
- Mulero, V., & Brock, J. H.
- Affiliations
- From the Department of Immunology and Bacteriology, Western Infirmary, University of Glasgow, Glasgow, UK; and the Department of Cell Biology, Faculty of Biology, University of Murcia, Murcia, Spain.
- Western Infirmary (GB)
- Venue
- Blood
- Publisher
- American Society of Hematology
- Published
- 1999-10-01
- Volume
- 94
- Issue
- 7
- Pages
- 2383-2389
- ISSN
- 0006-4971 (ISSN-L)
- 1528-0020
- OpenAlex
- W2144161937
- Cited by
- 86
- References
- 37
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 5 · 2020: 1 · 2018: 2 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 1 · 2012: 7
- FWCI
- 3.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Blood (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Hemoglobin structure and function
- Physical Sciences › Materials Science › Metals and Alloys › Iron and Steelmaking Processes
- Keywords
- nitric oxide, interferon-gamma, lipopolysaccharide, transferrin receptor, iron regulatory protein 1, IRP2, iron absorption, iron-responsive element, nitric oxide synthase, J774 macrophages
- MeSH terms
- Animals, Cell Line, Gene Expression Regulation, Interferon-gamma, Iron, Iron-Sulfur Proteins, Kinetics, Lipopolysaccharides, Macrophages, Nitric Oxide, Receptors, Transferrin, Recombinant Proteins, Transferrin, RNA-Binding Proteins, Nitric Oxide Synthase, omega-N-Methylarginine, Iron-Regulatory Proteins, Iron Regulatory Protein 1, Iron Regulatory Protein 2, Mice
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